지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Objectives: This study was designed to evaluate the macrophages polarization of traditional Korean medicine on cardiac pain about Geum-Gwe-Yo-Ryak"s two prescriptions including Kwaruhaebaekbanha-tang (KHB) and Kwaruhaebaekpaekju-tang (KHP).
Materials and methods: Flow cytometry analysis was used to measure the changes in the ratio of M1 type and M2 type macrophages. Protein expression of nuclear factor-like 2 (Nrf2), heme oxygenase-1 (HO-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were measured by Western Blot, and ABCA1 and SR-B1 were detected by real time PCR (RT-PCR). Intracellular lipid accumulation was measured by Oil Red O staining (ORO staining).
Results: KHB and KHP increase anti-oxidative activity related protein levels including Nrf2 and HO-1. Furthermore, KHB and KHP inhibit lipid accumulation on intracellular levels through induction of ATP binding receptor cassette subfamily A member 1 (ABCA1) and scavenging receptor class B member 1 (SR-B1), respectively. Finally, KHB and KHP also blocked pro-inflammatory mediators including tumor necrosis factor-alpha (TNFα) and interleukin-6 (IL-6), iNOS and COX-2 expression.
Conclusion: This study suggests that KHB and KHP potently regulate the M1/M2 macrophage polarization.
Materials and methods: Flow cytometry analysis was used to measure the changes in the ratio of M1 type and M2 type macrophages. Protein expression of nuclear factor-like 2 (Nrf2), heme oxygenase-1 (HO-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were measured by Western Blot, and ABCA1 and SR-B1 were detected by real time PCR (RT-PCR). Intracellular lipid accumulation was measured by Oil Red O staining (ORO staining).
Results: KHB and KHP increase anti-oxidative activity related protein levels including Nrf2 and HO-1. Furthermore, KHB and KHP inhibit lipid accumulation on intracellular levels through induction of ATP binding receptor cassette subfamily A member 1 (ABCA1) and scavenging receptor class B member 1 (SR-B1), respectively. Finally, KHB and KHP also blocked pro-inflammatory mediators including tumor necrosis factor-alpha (TNFα) and interleukin-6 (IL-6), iNOS and COX-2 expression.
Conclusion: This study suggests that KHB and KHP potently regulate the M1/M2 macrophage polarization.
목차
서론
재료 및 방법
결과 및 고찰
결론
참고문헌
참고문헌 (24)
참고문헌 신청
Tong X / 2019 / The burden of cerebrovascular disease in the United States / Prev Chronic Dis 16(52):180411
Terentes-Printzios D / 2017 / Cardiovascular risk factors accelerate progression of vascular aging in the general population : Results from CRAVE study(Cardiovascular Risk Factors Affecting Vascular Age) / Hypertension 70(5):1057~1064
John Hall / 2017 / Guyton and Hall Textbook of Medical Physiology / Bupmoon Education
Poupel L / 2010 / Atherosclerosis : on the trail of chemokines / Biol Aujourdhui 204(4):285~293
Munteanu AI / 2016 / Immunohistochemical study of the role of mast cells and macrophages in the process of angiogenesis in the atherosclerotic plaques in patients with metabolic syndrome / Arkh Patol 78(2):19~28
Terentes-Printzios D / 2017 / Cardiovascular risk factors accelerate progression of vascular aging in the general population : Results from CRAVE study(Cardiovascular Risk Factors Affecting Vascular Age) / Hypertension 70(5):1057~1064
John Hall / 2017 / Guyton and Hall Textbook of Medical Physiology / Bupmoon Education
Poupel L / 2010 / Atherosclerosis : on the trail of chemokines / Biol Aujourdhui 204(4):285~293
Munteanu AI / 2016 / Immunohistochemical study of the role of mast cells and macrophages in the process of angiogenesis in the atherosclerotic plaques in patients with metabolic syndrome / Arkh Patol 78(2):19~28
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UCI(KEPA) : I410-ECN-0101-2019-519-000946680