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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 188 - 196 (9page)
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Background/Aims: This study was conducted to identify microRNAs (miRNAs) that are differentially expressed in Helicobacter pylori-infected patients with an intestinal type of gastric cancer using miRNA microarray and to confirm the candidate miRNA expression levels. Methods: Total RNA was extracted from the cancerous and noncancerous regions of formalin-fixed, paraffin-embedded tissues of H. pylori-positive (n=8) or H. pylori-negative (n=8) patients with an intestinal type of gastric cancer. RNA expression was analyzed using a 3,523 miRNA profiling microarray based on the Sanger miRBase. Validation analysis was performed using TaqMan miRNA assays. Results: A total of 219 miRNAs in the aberrant miRNA profiles across the miRNA microarray showed at least a 2-fold change differential expression in H. pylori-positive and H. pylori-negative cancer tissues. After candidate miRNAs were selected using online miRNA databases, TaqMan miRNA assays confirmed that three miRNAs (miR-99b-3p, miR-564, and miR-638) were significantly increased in three H. pylori-positive cancer tissues compared to the H. pylori-negative cancer tissues. Additionally, four miRNAs (miR-204-5p, miR-338-5p, miR-375, and miR-548c-3p) were significantly increased in H. pylori-negative cancer tissues compared to H. pylori-positive cancer tissues. Conclusions: miRNA expression in the intestinal type of H. pylori infection-dependent gastric cancer suggests that different gastric cancer pathogenesis mechanisms could exist between H. pylori-positive and H. pylori-negative gastric cancer. Additional functional studies are required.
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참고문헌 (23)
참고문헌 신청
Ach RA / 2008 / Measuring microRNAs: comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods / BMC Biotechnol 8 : 69 ~
Ando T / 2009 / DNA methylation of microRNA genes in gastric mucosae of gastric cancer patients: its possible involvement in the formation of epigenetic fiel
Belair C / 2009 / Helicobacter pylori and gastric cancer: possible role of microRNAs in this intimate relationship / Clin Microbiol Infect 15 : 806 ~ 812
Dweep H / 2011 / miRWalk-database: prediction of possible miRNA binding sites by “walking” the genes of three genomes / J Biomed Inform 44 : 839 ~ 847
Git A / 2010 / Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression / RNA
Ando T / 2009 / DNA methylation of microRNA genes in gastric mucosae of gastric cancer patients: its possible involvement in the formation of epigenetic fiel
Belair C / 2009 / Helicobacter pylori and gastric cancer: possible role of microRNAs in this intimate relationship / Clin Microbiol Infect 15 : 806 ~ 812
Dweep H / 2011 / miRWalk-database: prediction of possible miRNA binding sites by “walking” the genes of three genomes / J Biomed Inform 44 : 839 ~ 847
Git A / 2010 / Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression / RNA
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