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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제52권 제3호
- 발행연도
- 2018.1
- 수록면
- 157 - 163 (7page)
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초록· 키워드
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Background: BRAF mutation has been recognized as an important biomarker of colorectal cancer (CRC) for targeted therapy and prognosis prediction. However, sequencing for every CRC case is not cost-effective. An antibody specific for BRAF V600E mutant protein has been introduced, and we thus examined the utility of BRAF VE1 immunohistochemistry for evaluating BRAF mutations in CRC. Methods: Fifty-one BRAF-mutated CRCs and 100 age and sexmatched BRAF wild-type CRCs between 2005 and 2015 were selected from the archives of Asan Medical Center. Tissue microarrays were constructed and stained with BRAF VE1 antibody. Results: Forty-nine of the 51 BRAF-mutant CRCs (96.1%) showed more than moderate cytoplasmic staining, except for two weakly stained cases. Six of 100 BRAF wild-type cases also stained positive with BRAF VE1 antibody; four stained weakly and two stained moderately. Normal colonic crypts showed nonspecific weak staining, and a few CRC cases exhibited moderate nuclear reactivity (3 BRAF-mutant and 10 BRAF wild-type cases). BRAF-mutated CRC patients had higher pathologic stages and worse survival than BRAF wild-type patients. Conclusions: BRAF VE1 immunohistochemistry showed high sensitivity and specificity, but occasional nonspecific staining in tumor cell nuclei and normal colonic crypts may limit their routine clinical use. Thus, BRAF VE1 immunohistochemistry may be a useful screening tool for BRAF V600E mutation in CRCs, provided that additional sequencing studies can be done to confirm the mutation in BRAF VE1 antibody-positive cases.
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참고문헌 (17)
참고문헌 신청
Barras D / 2015 / BRAF mutation in colorectal cancer: an update / Biomark Cancer 7(Suppl 1):9~12
Boulagnon C / 2016 / BRAFV600E gene mutation in colonic adenocarcinomas: immunohistochemical detection using tissue microarray and clinicopathologic characteristics: an 86 case series / Appl Immunohistochem Mol Morphol 24:88~96
Ritterhouse LL / 2015 / BRAF V600E mutation-specific antibody: a review / Semin Diagn Pathol 32:400~408
Safaee Ardekani G / 2012 / The prognostic value of BRAF mutation in colorectal cancer and melanoma: a systematic review and meta-analysis / PLoS One 7:e47054
Bloe JR / 2014 / BRAF V600E immunohistochemistry is reliable in primary and metastatic colorectal carcinoma regardless of treatment status and shows high intratumoral homogeneity / Am J Surg Pathol 38:1418~1428
Boulagnon C / 2016 / BRAFV600E gene mutation in colonic adenocarcinomas: immunohistochemical detection using tissue microarray and clinicopathologic characteristics: an 86 case series / Appl Immunohistochem Mol Morphol 24:88~96
Ritterhouse LL / 2015 / BRAF V600E mutation-specific antibody: a review / Semin Diagn Pathol 32:400~408
Safaee Ardekani G / 2012 / The prognostic value of BRAF mutation in colorectal cancer and melanoma: a systematic review and meta-analysis / PLoS One 7:e47054
Bloe JR / 2014 / BRAF V600E immunohistochemistry is reliable in primary and metastatic colorectal carcinoma regardless of treatment status and shows high intratumoral homogeneity / Am J Surg Pathol 38:1418~1428
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