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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 99 - 104 (6page)
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Background: Quantitation of cytomegalovirus (CMV) DNA using real-time PCR has been utilized for monitoring CMV infection. However, the CMV antigenemia assay is still the ‘gold standard’ assay. There are only a few studies in Korea that compared the efficacy of use of real-time PCR for quantitation of CMV DNA in whole blood with the antigenemia assay, and most of these studies have been limited to transplant recipients.
Method: 479 whole blood samples from 79 patients, falling under different disease groups, were tested by real-time CMV DNA PCR using the Q-CMV real-time complete kit (Nanogen Advanced Diagnostic S.r.L., Italy) and CMV antigenemia assay (CINA Kit, ArgeneBiosoft, France), and the results were compared. Repeatedly tested patients were selected and their charts were reviewed for ganciclovir therapy.
Results: The concordance rate of the two assays was 86.4% (Cohen’s kappa coefficient value=0.659). Quantitative correlation between the two assays was a moderate (r=0.5504, P<0.0001). Among 20 patients tested repeatedly with the two assays, 13 patients were transplant recipients and treated with ganciclovir. Before treatment, CMV was detected earlier by real-time CMV DNA PCR than the antigenemia assay, with a median difference of 8 days. After treatment, the antigenemia assay achieved negative results earlier than real-time CMV DNA PCR with a median difference of 10.5 days.
Conclusions: Q-CMV real-time complete kit is a useful tool for early detection of CMV infection in whole blood samples in transplant recipients.
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참고문헌 (23)
참고문헌 신청
Pillet S / 2014 / Quantification of cytomegalovirus viral load / Expert Rev Anti Infect Ther 12:193~210
Kim DJ / 2007 / Real-time PCR assay compared with antigenemia assay for detecting cytomegalovirus infection in kidney transplant recipients / Transplant Proc 39:1458~1460
Rhee JY / 2011 / Clinical usefulness of plasma quantitative polymerase chain reaction assay: diagnosis of cytomegalovirus infection in kidney transplant recipients / Transplant Proc 43:2624~2629
Allice T / 2008 / Evaluation of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp65-antigen test in guiding pre-emptive
Boeckh M / 1998 / Quantitation of cytomegalovirus: methodologic aspects and clinical applications / Clin Microbiol Rev 11 : 533 ~ 554
Kim DJ / 2007 / Real-time PCR assay compared with antigenemia assay for detecting cytomegalovirus infection in kidney transplant recipients / Transplant Proc 39:1458~1460
Rhee JY / 2011 / Clinical usefulness of plasma quantitative polymerase chain reaction assay: diagnosis of cytomegalovirus infection in kidney transplant recipients / Transplant Proc 43:2624~2629
Allice T / 2008 / Evaluation of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp65-antigen test in guiding pre-emptive
Boeckh M / 1998 / Quantitation of cytomegalovirus: methodologic aspects and clinical applications / Clin Microbiol Rev 11 : 533 ~ 554
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