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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 226 - 235 (10page)
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Purpose: Long non-coding RNA taurine upregulated gene 1 (TUG1) is reported to be a vital regulator of the progression of various cancers. This study aimed to explore the exact roles and molecular mechanisms of TUG1 in osteosarcoma (OS) development. Materials and Methods: Real-time quantitative PCR was applied to detect the expressions of TUG1 and microRNA-132-3p (miR-132-3p) in OS tissues and cells. Western blot was performed to measure protein levels of sex determining region Y-box 4 (SOX4). Cell viability was assessed using XTT assay. Cell apoptosis was evaluated using flow cytometry and caspase-3 activity detection assays. Bioinformatics analysis and luciferase reporter experiments were employed to confirm relationships among TUG1, miR-132-3p, and SOX4. Results: TUG1 was highly expressed in human OS tissues, OS cell lines, and primary OS cells. TUG1 knockdown hindered proliferationand induced apoptosis in human OS cell lines and primary OS cells. Moreover, TUG1 inhibited miR-132-3p expression by direct interaction, and introduction of miR-132-3p inhibitor partly abrogated the effect of TUG1 knockdown on the proliferation and apoptosis of OS cells. Furthermore, SOX4 was validated as a target of miR-132-3p. Further functional analyses revealed that miR-132-3p inhibited proliferation and induced apoptosis of OS cells, while this effect was greatly abated following SOX4 overexpression. Moreover, TUG1 knockdown suppressed proliferation and promoted apoptosis by upregulating miR-132-3p and downregulatingSOX4 in primary OS cells. Conclusion: TUG1 facilitated proliferation and suppressed apoptosis by regulating the miR-132-3p/SOX4 axis in human OS cell lines and primary OS cells. This finding provides a potential target for OS therapy.
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참고문헌 (30)
참고문헌 신청
Mirabello L / 2009 / Osteosarcoma incidence and survival rates from 1973 to 2004: data from the Surveillance, Epidemiology, and End Results Program / Cancer 115:1531~1543
Meyers PA / 2008 / Osteosarcoma: the addition of muramyl tripep- tide to chemotherapy improves overall survival--a report from the Children's Oncology Group / J Clin Oncol 26:633~638
Esteller M / 2011 / Non-coding RNAs in human disease / Nat Rev Genet 12:861~874
Catto JW / 2011 / MicroRNA in prostate, bladder, and kidney cancer: a systematic review / Eur Urol 59:671~681
Yang G / 2014 / LncRNA: a link between RNA and cancer / Biochim Biophys Acta 1839:1097~1109
Meyers PA / 2008 / Osteosarcoma: the addition of muramyl tripep- tide to chemotherapy improves overall survival--a report from the Children's Oncology Group / J Clin Oncol 26:633~638
Esteller M / 2011 / Non-coding RNAs in human disease / Nat Rev Genet 12:861~874
Catto JW / 2011 / MicroRNA in prostate, bladder, and kidney cancer: a systematic review / Eur Urol 59:671~681
Yang G / 2014 / LncRNA: a link between RNA and cancer / Biochim Biophys Acta 1839:1097~1109
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