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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 349 - 357 (9page)
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Purpose: This study was designed to investigate the role of taurine- upregulated gene 1 (TUG1) in MCF-7 breast cancer cells and the molecular mechanism involved in the regulation of microRNA- 9 (miR-9). Methods: The expression of TUG1 in breast cancer tissues and cells was evaluated using quantitative reverse transcription polymerase chain reaction. Cell viability was examined using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide (MTT) assay; cell cycle progression and apoptosis were analyzed using flow cytometry. A dual luciferase reporter assay was used to detect the relationship between TUG1 and miR-9. The expression of methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was measured by western blot. Results: Higher expression of TUG1 was observed in breast cancer tissues and cell lines than in the corresponding controls. TUG1 knockdown reduced proliferation, suppressed cell cycle progression, and promoted apoptosis of MCF-7 cells. The dual luciferase reporter assay showed that TUG1 could negatively regulate the expression of miR-9. MiR-9 inhibition abrogated the effect of TUG1 knockdown on the proliferation, cell cycle progression, and apoptosis of MCF-7 cells. TUG1 positively regulated the expression of MTHFD2 in breast cancer cells. Conclusion: TUG1 knockdown was significantly associated with decreased cell proliferation and it promoted apoptosis of breast cancer cells through the regulation of miR-9.
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참고문헌 (29)
참고문헌 신청
Yan S / 2015 / Prognostic significance of c-Met in breast cancer: a meta-analysis of 6010 cases / Diagn Pathol 10:62
O’Brien KM / 2015 / Hormone therapy and young-onset breast cancer / Am J Epidemiol 181:799~807
Shuvalov O / 2015 / Current genome editing tools in gene therapy: new approaches to treat cancer / Curr Gene Ther 15:511~529
Gibb EA / 2011 / The functional role of long non-coding RNA in human carcinomas / Mol Cancer 10:38
Xing Z / 2015 / LncRNA BCAR4 wires up signaling transduction in breast cancer / RNA Biol 12:681~689
O’Brien KM / 2015 / Hormone therapy and young-onset breast cancer / Am J Epidemiol 181:799~807
Shuvalov O / 2015 / Current genome editing tools in gene therapy: new approaches to treat cancer / Curr Gene Ther 15:511~529
Gibb EA / 2011 / The functional role of long non-coding RNA in human carcinomas / Mol Cancer 10:38
Xing Z / 2015 / LncRNA BCAR4 wires up signaling transduction in breast cancer / RNA Biol 12:681~689
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