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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 38 - 46 (9page)
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Cholesterol is one of major components of cell membrane and plays a role in vesicular trafficking and cellular signaling. We investigated the effects of cholesterol on matrix metalloproteinase-2 (MMP-2) activation in human dermal fibroblasts. We found that tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) expression and active form MMP-2 (64 kD) were dose-dependently increased by methyl-β-cyclodextrin (MβCD), a cholesterol depletion agent. In contrast, cholesterol depletion-induced TIMP-2 expression and MMP-2 activation were suppressed by cholesterol repletion. Then we investigated the regulatory mechanism of TIMP-2expression by cholesterol depletion. We found that the phosphorylation of JNK as well as ERK was significantly increased by cholesterol depletion.
Moreover, cholesterol depletion-induced TIMP-2 expression and MMP-2 activation was significantly decreased by MEK inhibitor U0126, and JNK inhibitor SP600125, respectively. While a low dose of recombinant TIMP-2 (100 ng/ml) increased the level of active MMP-2 (64 kD), the high dose of TIMP-2 (≥ 200 ng/ml)decreased the level of active MMP-2 (64 kD). Taken together,we suggest that the induction of TIMP-2 by cholesterol depletion leads to the conversion of proMMP-2(72 kD) into active MMP-2 (64 kD) in human dermal fibroblasts.
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참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)]
Atkinson SJ / 2004 / Cellular cholesterol regulates MT1 MMP dependent activation of MMP 2 via MEK-1 in HT1080 fibrosarcoma cells / FEBS Lett 566 : 65 ~ 70
Chung JH / 2001 / Modulation of skin collagen metabolism in aged and photoaged human skin in vivo / J Invest Dermatol 117 : 1218 ~ 1224
Ellerbroek SM / 1999 / Membrane associated matrix metalloproteinases in metastasis / Bioessays 21 : 940 ~ 949
Elyassaki W / 2006 / Lipid rafts mediate ultraviolet light-induced Fas aggregation in M624 melanoma cells / Photochem Photobiol 82 : 787 ~ 792
Atkinson SJ / 2004 / Cellular cholesterol regulates MT1 MMP dependent activation of MMP 2 via MEK-1 in HT1080 fibrosarcoma cells / FEBS Lett 566 : 65 ~ 70
Chung JH / 2001 / Modulation of skin collagen metabolism in aged and photoaged human skin in vivo / J Invest Dermatol 117 : 1218 ~ 1224
Ellerbroek SM / 1999 / Membrane associated matrix metalloproteinases in metastasis / Bioessays 21 : 940 ~ 949
Elyassaki W / 2006 / Lipid rafts mediate ultraviolet light-induced Fas aggregation in M624 melanoma cells / Photochem Photobiol 82 : 787 ~ 792
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