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W/O/W (water-in-oil-in-water) type multiple emulsionwas applied to improve the storage stability of an antagonisticmicroorganism, Burkholderia gladioli. Encapsulation ofmicroorganism into a W/O/W emulsion was conducted byusing a two-step emulsification method. W/O/W emulsionwas prepared by the incorporation of B. gladioli into rapeseedoil and the addition of polyglycerin polyriconolate (PGPR)and castor oil polyoxyethylene (COG 25) as the primary andsecondary emulsifier, respectively. Microcrystalline cellulosewas used as an emulsion stabilizer. To evaluate the usefulnessof W/O/W emulsion formulation as a microbial pesticide forcontrolling the bacterial wilt pathogen (Ralstonia solanacearum),the storage stability and antagonistic activity of emulsionformulation were tested in vitro. The storage stability testrevealed that the viability of formulated cells in emulsion washigher than that of unformulated cells in culture broth. At 4oC,the viabilities of formulated cells and unformulated cells atthe end of 20 weeks decreased to about 2 and 5 log cycles,respectively. At 37oC, the viability of formulated cells decreasedto only 2 log cycles at the end of storage. On the other hand,the viable cells in culture broth were not detected after 13weeks. In activity test, formulated cells in emulsion weremore effective in inhibiting the growth of pathogen thanunformulated cells in culture broth. Unformulated cells completelylost their antagonistic activity during storage under similarconditions. The W/O/W multiple emulsion formulation wasshown to be useful as the novel liquid formulation forbiological control.

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