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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제1호
- 발행연도
- 2008.1
- 수록면
- 183 - 187 (5page)
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Vascular endothelial growth factors (VEGFs) are a family of proteins that mediate angiogenesis. VEGF165 is a VEGF-A isoform and has been extensively studied owing to its potential use in therapeutic angiogenesis. This study established Chinese hamster ovary (CHO) cells overexpressing recombinant human VEGF165 (rhVEGF165) protein. The production rate of the established CHO cells was over 80 mg/l of rhVEGF165 protein from a 7-day batch culture process using a 7.5-l bioreactor with a 5-l working volume and serum-free medium. The rhVEGF165 protein was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure that resulted in a 48% recovery rate. The purified rhVEGF165 protein was a glycosylated homodimeric protein with a higher molecular weight (MW) than the protein expressed from insect cells, suggesting that the glycosylation of the rhVEGF165 protein in CHO cells differed from that in insect cells. The purified rhVEGF165 protein in this study was functionally active with a half-maximal effective concentration of 3.8 ng/ml and specific activity of 2.5×105 U/mg.
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참고문헌 (24)
참고문헌 신청
[학술지(정기간행
Bae, G. W. / 2006 / High productivity of t-PA in CHO cells using hypoxia response element. J. Microbiol / Biotechnol 16 : 695 ~ 703
Bhisitkul, R. B. / 2006 / Vascular endothelial growth factor biology: Clinical implications for ocular treatments. Br / Ophthalmol 90 : 1542 ~ 1547
Byun, J. / 2001 / Efficient expression of the vascular endothelial growth factor gene in vitro and in vivo, using an adeno-associated virus vector. J. Mol. Cell / Cardiol 33
Carmeliet, P. / 2003 / Angiogenesis in health and disease. Nat / Med 9 : 653 ~ 660
Bae, G. W. / 2006 / High productivity of t-PA in CHO cells using hypoxia response element. J. Microbiol / Biotechnol 16 : 695 ~ 703
Bhisitkul, R. B. / 2006 / Vascular endothelial growth factor biology: Clinical implications for ocular treatments. Br / Ophthalmol 90 : 1542 ~ 1547
Byun, J. / 2001 / Efficient expression of the vascular endothelial growth factor gene in vitro and in vivo, using an adeno-associated virus vector. J. Mol. Cell / Cardiol 33
Carmeliet, P. / 2003 / Angiogenesis in health and disease. Nat / Med 9 : 653 ~ 660
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