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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,801 - 1,809 (9page)
이용수
초록· 키워드
오류제보하기
A phytoene synthase gene, crtB, was isolated from Kocuria gwangalliensis. The crtB with 1,092 bp full-length has a coding sequence of 948 bp and encodes a 316-amino-acids protein. The deduced amino acid sequence showed a 70.9% identity with a putative phytoene synthase from K. rhizophila. An expression plasmid, pCcrtB, containing the crtB gene was constructed, and E. coli cells containing this plasmid produced the recombinant protein of approximately 34kDa , corresponding to the molecular mass of phytoene synthase. Biosynthesis of lycopene was confirmed when the plasmid pCcrtB was co-transformed into E. coli containing pRScrtEI carrying the crtE and crtI genes encoding lycopene biosynthetic pathway enzymes. The results obtained from this study will provide a base of knowledge about the phytoene synthase of K. gwangalliensis and can be applied to the production of carotenoids in a non-carotenoidproducing host.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Ashby MN / 1990 / Molecular Biology of Atherosclerosis / Elsevier : 27 ~ 34
Bartley GE / 1993 / cDNA cloning, expression during development, and genome mapping of PSY2, a second tomato gene encoding phytoene synthase / J. Biol. Chem 268 : 25718 ~ 257
Bramley PM / 1988 / Current Topics in Cellular Regulation, Vol. 29 / Academic Press : 291 ~ 343
Bubrick P. / 1991 / Production of astaxanthin from Haematococcus / Bioresour. Technol 38 : 237 ~ 239
Collins MD / 1983 / Classification of some coryneform bacteria in a new genus Aureobacterium / Syst. Appl. Microbiol 4 : 236 ~ 252
Bartley GE / 1993 / cDNA cloning, expression during development, and genome mapping of PSY2, a second tomato gene encoding phytoene synthase / J. Biol. Chem 268 : 25718 ~ 257
Bramley PM / 1988 / Current Topics in Cellular Regulation, Vol. 29 / Academic Press : 291 ~ 343
Bubrick P. / 1991 / Production of astaxanthin from Haematococcus / Bioresour. Technol 38 : 237 ~ 239
Collins MD / 1983 / Classification of some coryneform bacteria in a new genus Aureobacterium / Syst. Appl. Microbiol 4 : 236 ~ 252
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