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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제2호
- 발행연도
- 2016.1
- 수록면
- 394 - 401 (8page)
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Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) system, a genome editing technology, was shown to be versatile in treating several antibiotic-resistant bacteria. In the present study, we applied the CRISPR/ Cas9 technology to kill extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. ESBL bacteria are mostly multidrug resistant (MDR), and have plasmid-mediated antibiotic resistance genes that can be easily transferred to other members of the bacterial community by horizontal gene transfer. To restore sensitivity to antibiotics in these bacteria, we searched for a CRISPR/Cas9 target sequence that was conserved among >1,000 ESBL mutants. There was only one target sequence for each TEM- and SHV-type ESBL, with each of these sequences found in ~200 ESBL strains of each type. Furthermore, we showed that these target sequences can be exploited to re-sensitize MDR cells in which resistance is mediated by genes that are not the target of the CRISPR/Cas9 system, but by genes that are present on the same plasmid as target genes. We believe our Re-Sensitization to Antibiotics from Resistance (ReSAFR) technology, which enhances the practical value of the CRISPR/Cas9 system, will be an effective method of treatment against plasmid-carrying MDR bacteria.
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참고문헌 (24)
참고문헌 신청
Abraham EP / 1940 / An enzyme from bacteria able to destroy penicillin / Rev. Infect. Dis 10:677~678
Bhaya D / 2011 / CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation / Annu. Rev. Genet 45:273~297
Bikard D / 2014 / Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials / Nat. Biotechnol 32:1146~1150
Butler MS / 2013 / Antibiotics in the clinical pipeline in 2013 / J. Antibiot 66:571~591
Citorik RJ / 2014 / Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases / Nat. Biotechnol 32:1141~1145
Bhaya D / 2011 / CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation / Annu. Rev. Genet 45:273~297
Bikard D / 2014 / Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials / Nat. Biotechnol 32:1146~1150
Butler MS / 2013 / Antibiotics in the clinical pipeline in 2013 / J. Antibiot 66:571~591
Citorik RJ / 2014 / Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases / Nat. Biotechnol 32:1141~1145
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