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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 39 - 45 (7page)
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Stem cell therapy using mesenchymal stem cells(MSCs) transplantation have been paid attention because of their powerful proliferation and pluripotent differentiating ability. Although umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, the presence of mesenchymal stem cells (MSCs) in UCB has been controversial and it remains to be validated. In this study, we examine the presence of MSCs in UCB harvests and the prevalence of them is compared to that of endothelial progenitor cells. For this, CD34+ and CD34- cells were isolated and cultured under the endothelial cell growth medium and mesenchymal stem cell growth medium respectively. The present study showed that ESC-like cells could be isolated and expanded from preterm UCBs but were not acquired efficiently from full-terms. They expressed CD14-, CD34-, CD45-, CD29+, CD44+, CD105+ cell surface marker and could differentiate into adipogenic and osteogenic lineages. Our results suggest that MSCs are fewer in full-term UCB compared to endothelial progenitor cells.
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참고문헌 (16)
참고문헌 신청
/ / Alternative source of neurons and glia from somatic stem cells Cell Transplant 2002 : 25 ~ 34
/ / Growth kinetics, self renewaland the osteogenic potential of purified human mesenchymal stem cells during extensive subcultuivation and following cryopreservation J Cell
/ / High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years Proc Natl Acad Sci USA 2003 : 645 ~ 650
/ / Human cord blood-derived cells attain neuronal and glial features in vitro J Cell Sci 2002 : 2131 ~ 2138
/ / Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood campag (li c) : 2396 ~ 2402
/ / Growth kinetics, self renewaland the osteogenic potential of purified human mesenchymal stem cells during extensive subcultuivation and following cryopreservation J Cell
/ / High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years Proc Natl Acad Sci USA 2003 : 645 ~ 650
/ / Human cord blood-derived cells attain neuronal and glial features in vitro J Cell Sci 2002 : 2131 ~ 2138
/ / Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood campag (li c) : 2396 ~ 2402
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