지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 360 - 365 (6page)
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Purpose: To investigate the effects of anti-vascular endothelial growth factor (VEGF) antibody on the survival ofretinal ganglion cell (RGC)-5 cells differentiated with staurosporine under oxidative stress.
Methods: We used real-time polymerase chain reaction and Western blot to confirm the expression of VEGF,VEGF receptor (VEGFR)-1 and VEGFR-2 in RGC-5 cells differentiated with staurosporine for 6 hours. The differentiatedRGC-5 cells were treated with 800 μM hydrogen peroxide (H2O2) for 24 hours to induce oxidativestress. Then, the survival rate of RGC-5 was confirmed by lactate dehydrogenase assay at each concentration(0, 0.01, 0.1, and 1 mg) using bevacizumab as the anti-VEGF antibody. The expression of VEGF, VEGFR-1,and VEGFR-2 was confirmed using real-time polymerase chain reaction.
Results: VEGF, VEGFR-1, and VEGFR-2 were all expressed in differentiated RGC-5 cells. When RGC-5 cellswere simultaneously treated with bevacizumab and 800 μM H2O2, survival of RGC-5 decreased with bevacizumabconcentration. VEGF expression in RGC-5 cells increased with increasing concentration of bevacizumab.
Similar patterns were observed for VEGFR-1 and VEGFR-2, but the degree of increase was smaller thanthat for VEGF.
Conclusions: When bevacizumab was administered to differentiated RGC-5 cells, the cell damage caused byoxidative stress increased. Therefore, given these in vitro study results, caution should be exercised with bevacizumabtreatment.
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참고문헌 (22)
참고문헌 신청
Adamis AP / 1994 / Increased vascular endothelial growth factor levels in the vitreous of eyes with proliferative diabetic retinopathy / Am J Ophthalmol 118 : 445 ~ 450
Aiello LP / 1994 / Vascular endothelial growth factor in ocular fluid of patients with diabetic retinopathy and other retinal disorders / N Engl J Med 331 : 1480 ~ 1487
Beatty S / 2000 / The role of oxidative stress in the pathogenesis of age-related macular degeneration / Surv Ophthalmol 45 : 115 ~ 134
Charles I / 2005 / Serum deprivation induces apoptotic cell death of transformed rat retinal ganglion cells via mitochondrial signaling pathways / Invest Ophthalmol Vis Sci
Chen SD / 2013 / Neuroprotection in glaucoma:present and future / Chin Med J (Engl) 126 : 1567 ~ 1577
Aiello LP / 1994 / Vascular endothelial growth factor in ocular fluid of patients with diabetic retinopathy and other retinal disorders / N Engl J Med 331 : 1480 ~ 1487
Beatty S / 2000 / The role of oxidative stress in the pathogenesis of age-related macular degeneration / Surv Ophthalmol 45 : 115 ~ 134
Charles I / 2005 / Serum deprivation induces apoptotic cell death of transformed rat retinal ganglion cells via mitochondrial signaling pathways / Invest Ophthalmol Vis Sci
Chen SD / 2013 / Neuroprotection in glaucoma:present and future / Chin Med J (Engl) 126 : 1567 ~ 1577
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