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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제6호
- 발행연도
- 2019.1
- 수록면
- 887 - 896 (10page)
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초록· 키워드
오류제보하기
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based pathogen identification relies on the ribosomal protein spectra provided in the proprietary database. Although these mass spectra can discern various pathogens at species level, the spectra-based method still has limitations in identifying closely-related microbial species. In this study, to overcome the limits of the current MALDI-TOF MS identification method using ribosomal protein spectra, we applied MALDI-TOF MS of low-mass profiling to the identification of two genetically related Bacillus species, the food-borne pathogen Bacillus cereus, and the insect pathogen Bacillus thuringiensis. The mass spectra of small molecules from 17 type strains of two bacilli were compared to the morphological, biochemical, and genetic identification methods of pathogens. The specific mass peaks in the low-mass range (m/z 500– 3,000) successfully identified various closely-related strains belonging to these two reference species. The intensity profiles of the MALDI-TOF mass spectra clearly revealed the differences between the two genetically-related species at strain level. We suggest that small molecules with low molecular weight, 714.2 and 906.5 m/z can be potential mass biomarkers used for reliable identification of B. cereus and B. thuringiensis.
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등록된 정보가 없습니다.
참고문헌 (54)
참고문헌 신청
Barbuddhe SB / 2008 / Rapid identification and typing of listeria species by matrix-assisted laser desorption ionizationtime of flight mass spectrometry / Appl. Environ. Microbiol 74:5402~5407
Bessède E / 2011 / Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight(MALDI-TOF)mass spectrometry / Clin. Microbiol. Infect 17:1735~1739
Bizzini A / 2010 / Performance of matrix-assisted laser desorption ionizationtime of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory / J. Clin. Microbiol 48:1549~1554
Dieckmann R / 2010 / Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry / J. Appl. Microbiol 109:199~211
Grosse-Herrenthey A / 2008 / Challenging the problem of clostridial identification with matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry(MALDI-TOF MS) / Anaerobe 14:242~249
Bessède E / 2011 / Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight(MALDI-TOF)mass spectrometry / Clin. Microbiol. Infect 17:1735~1739
Bizzini A / 2010 / Performance of matrix-assisted laser desorption ionizationtime of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory / J. Clin. Microbiol 48:1549~1554
Dieckmann R / 2010 / Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry / J. Appl. Microbiol 109:199~211
Grosse-Herrenthey A / 2008 / Challenging the problem of clostridial identification with matrix-assisted laser desorption and ionization-time-of-flight mass spectrometry(MALDI-TOF MS) / Anaerobe 14:242~249
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