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논문 기본 정보

자료유형
학술저널
저자정보
Abhik Mojumdar (Korea Basic Science Institute (KBSI)) Hee-Jin Yoo (Korea Basic Science Institute (KBSI)) Duck-Hyun Kim (Korea Basic Science Institute (KBSI)) Kun Cho (Korea Basic Science Institute (KBSI))
저널정보
한국질량분석학회 Mass Spectrometry Letters Mass Spectrometry Letters Vol.13 No.4
발행연도
2022.12
수록면
106 - 114 (9page)

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초록· 키워드

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A rapid and reliable approach to the identification of microorganisms is a critical requirement for large-scale culturomics analysis. MALDI-TOF MS is a suitable technique that can be a better alternative to conventional biochemical and gene sequencing methods as it is economical both in terms of cost and labor. In this review, the applications of MALDI-TOF MS for the comprehensive identification of microorganisms and bacterial strain typing for culturomics-based approaches for various environmental studies including bioremediation, plant sciences, agriculture and food microbiology have been widely explored. However, the restriction of this technique is attributed to insufficient coverage of the mass spectral database. To improve the applications of this technique for the identification of novel isolates, the spectral database should be updated with the peptide mass fingerprint (PMF) of type strains with not only microbes with clinical relevance but also from various environmental sources. Further, the development of enhanced sample processing methods and new algorithms for automation and de-replication of isolates will increase its application in microbial ecology studies.

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Abstract
Introduction
MALDI-TOF MS in microbial identification
Conclusions
References

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