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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 762 - 769 (8page)
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Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK- 7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.
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참고문헌 (24)
참고문헌 신청
Walther B / 2013 / Menaquinones, bacteria, and the food supply : the relevance of dairy and fermented food products to vitamin K requirements / Adv. Nutr 4:463~473
Mahdinia E / 2017 / Optimization of Bacillus subtilis natto growth parameters in glycerol-based medium for vitamin K(menaquinone-7)production in biofilm reactors / Bioprocess Biosyst. Eng 41:195~204
Scheiber D / 2015 / High-dose menaquinone-7 supplementation reduces cardiovascular calcification in a murine model of extraosseous calcification / Nutrients 7:6991~7011
El Asmar MS / 2014 / Vitamin k dependent proteins and the role of vitamin K2 in the modulation of vascular calcification : a review / Oman. Med. J 29:172~177
Ma Y / 2019 / Metabolic engineering of the MEP pathway in Bacillus subtilis for increased biosynthesis of menaquinone-7 / ACS Synth. Biol 8:1620~1630
Mahdinia E / 2017 / Optimization of Bacillus subtilis natto growth parameters in glycerol-based medium for vitamin K(menaquinone-7)production in biofilm reactors / Bioprocess Biosyst. Eng 41:195~204
Scheiber D / 2015 / High-dose menaquinone-7 supplementation reduces cardiovascular calcification in a murine model of extraosseous calcification / Nutrients 7:6991~7011
El Asmar MS / 2014 / Vitamin k dependent proteins and the role of vitamin K2 in the modulation of vascular calcification : a review / Oman. Med. J 29:172~177
Ma Y / 2019 / Metabolic engineering of the MEP pathway in Bacillus subtilis for increased biosynthesis of menaquinone-7 / ACS Synth. Biol 8:1620~1630
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