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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 12 - 18 (7page)
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Microbial strains exhibiting proteolytic activity were isolated from kimchi, one of traditional fermented foods in Korea. Eight strains formed clear zones around their colonies when grown on TSA plates supplemented with skim milk. MBE/L865 exhibited 2.6-fold higher protease activity than that of control strain (Bacillus subtilis KCTC13112). MBE/L865 was identified as B. subtilis and deposited in the Korean Collection for Type Cultures under the accession number of KCCM43059. The optimum growth conditions for B. subtilis KCCM43059 were determined to be 37℃ and pH 8. The strain showed maximum protease activity (429.37 ± 18.65 U/mg protein) at 60℃ and pH 6. Further, B. subtilis KCCM43059 had a higher salt (NaCl) tolerance than that of the control strain.
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참고문헌 (26)
참고문헌 신청
Banerjee R / 1993 / Kinetic properties of extracellular alkaline protease of Rhizopus oryzae / J. Ferment. Bioeng 75 : 380 ~ 382
Banerjee U / 1999 / Thermostable alkaline protease from Bacillus brevis and its characterization as a laundary detergent additive / Process. Biochem 35 : 213 ~ 219
Banik R / 2004 / Laundry detergent compatibility of the alkaline protease from Bacillus cereus / Microbiol. Res 159 : 135 ~ 140
Dhandapani R / 1994 / Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4 / World. J. Microbiol. Biotechnol 10 : 33 ~ 35
Eiggert T / 2003 / Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH / FEMS Microbiol. Let
Banerjee U / 1999 / Thermostable alkaline protease from Bacillus brevis and its characterization as a laundary detergent additive / Process. Biochem 35 : 213 ~ 219
Banik R / 2004 / Laundry detergent compatibility of the alkaline protease from Bacillus cereus / Microbiol. Res 159 : 135 ~ 140
Dhandapani R / 1994 / Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4 / World. J. Microbiol. Biotechnol 10 : 33 ~ 35
Eiggert T / 2003 / Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH / FEMS Microbiol. Let
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