지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 429 - 437 (9page)
이용수
초록· 키워드
오류제보하기
ObjectiveThe male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgicallyretrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration (PESA), arediscussed and studied in men aged ≤38 years to achieve successful conception using assisted reproductive technology. The purpose was to assess the fertilization rate (FA), clinical pregnancy, and live birth rate (LBR) with sperm. MethodsA total of 287 semen samples were divided into 4 groups as follows: fresh PESA (n=73), frozen PESA (n=65), fresh TESA(n=128), and frozen TESA (n=21). The DNA fragmentation test using sperm chromatin dispersion assay was measuredand reported. ResultsFA was 70.3% and 65.5%, (P<0.022) for fresh and frozen epididymal sperm and 53.8% and 49.5%, (P<0.032) for freshand frozen testicular sperm. LBR was 33.6% and 30.2% (P<0.075) for fresh and frozen epididymal sperm (PESA) and22.7% and 18.2% (P<0.063) for fresh and frozen-thawed TESA sperm. ConclusionExposure to tissue shearing may adversely affect sperm quality. Increased sperm DNA damage due to long-termexposure while teasing enhances reactive oxygen species production foremost to membrane damage because ofthe oxidation of polyunsaturated fatty acid in lipids (lipid peroxidation), oxidation of amino acid in proteins, andinactivation of specific enzymes, all leading to enzymatic dipping and possibility of less fertilization and conception asindicated by the increase in LBR with fresh/frozen PESA compared to with fresh/frozen TESA.
목차
등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Temple-Smith PD / 1985 / Human pregnancy by in vitro fertilization(IVF)using sperm aspirated from the epididymis / J In Vitro Fert Embryo Transf 2:119~122
Corea M / 2005 / The diagnosis of azoospermia depends on the force of centrifugation / Fertil Steril 83:920~922
Esteves SC / 2013 / Reproductive potential of men with obstructive azoospermia undergoing percutaneous sperm retrieval and intracytoplasmic sperm injection according to the cause of obstruction / J Urol 189:232~237
Glina S / 2003 / Percutaneous epididymal sperm aspiration(PESA)in men with obstructive azoospermia / Int Braz J Urol 29:141~145
Hao L / 2017 / Application of percutaneous epididymal sperm aspiration in azoospermia / Eur Rev Med Pharmacol Sci 21:1032~1035
Corea M / 2005 / The diagnosis of azoospermia depends on the force of centrifugation / Fertil Steril 83:920~922
Esteves SC / 2013 / Reproductive potential of men with obstructive azoospermia undergoing percutaneous sperm retrieval and intracytoplasmic sperm injection according to the cause of obstruction / J Urol 189:232~237
Glina S / 2003 / Percutaneous epididymal sperm aspiration(PESA)in men with obstructive azoospermia / Int Braz J Urol 29:141~145
Hao L / 2017 / Application of percutaneous epididymal sperm aspiration in azoospermia / Eur Rev Med Pharmacol Sci 21:1032~1035
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