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논문 기본 정보

자료유형
학술저널
저자정보
윤혜균 (건국대학교, 동물자원연구센터) 윤산현 (마리아병원 불임클리닉) 임진호 (마리아병원 불임클리닉) 이훈택 (건국대학교, 동물자원연구센터) 정길생 (건국대학교, 동물자원연구센터)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제18권 제1호
발행연도
1994.1
수록면
71 - 81 (11page)

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초록· 키워드

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These experiments were carried out to investigate the effects of human follicular fluid (hFF) as a protein supplement on development of mammalian embryo as well as to find out ways toward effective use of hFF. The developmental rates of mouse embryos to the blastocyst and implantation stages were significantly higher in T6 +hFF than T6+hFCS. Classified hFF according to the maturity of contained oocytes (M-hFF and Im-hFF), and compared the rates of development of mouse embryo cultured in M-hFF or Im-hFF to culture medium T6. Total protein, albumin and estradiol concentrations were higher in M-hFF than Im-hFF (P<0.05). The developmental rates of mouse embryos to the blastocyst and hatching blastocyst stages cultured in Im-hFF were significantly lower than those in M-hFF and the basic medium. In accordance of the results of human IVF, hFF has been divided into 4 groups. The developmental rates of mouse embryos to the blastocyst stage in presense of hFF from pregnant patients, who have good grade embryos, were significantly higher than those in hFF from patients who have poor grade embryos or were not pregnant. In addition, the rates of development of human embryo were compared in presense of BSA, hFF or hFCS. The developmental rates of human embryos cultured in Ham's F10+hFF were significantly higher than those in the Ham's F10+BSA. These results suggests that the culture system using hFF could improve the development ability of mammalian embryos and the viability of blastocysts cultured in vitro.

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