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논문 기본 정보

자료유형
학술저널
저자정보
Shuang Liang (Yangzhou University) Zhiyuan Bao (Yangzhou University) Bohao Zhao (Yangzhou University) Tong Zhou (Yangzhou University) Jiali Li (Yangzhou University) Ming Liu (Yangzhou University) Shuaishuai Hu (Yangzhou University) Naisu Yang (Yangzhou University) Yang Chen (Yangzhou University) Xinsheng Wu (Yangzhou University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.11
발행연도
2020.1
수록면
1,281 - 1,290 (10page)

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Background Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determiningthe physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1in hair follicle development have so far not been reported. Objective This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development. Methods Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors andinterference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues,and the effects on other hair follicle development-related genes. Results The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localizedmostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, itwas found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally duringhair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2,KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P < 0.05), the converse after the corresponding useof si-RNA interference. Conclusions Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicledevelopment and Krtap11-1 gene expression.

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