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논문 기본 정보

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학술저널
저자정보
Chan Yeong Heo (Seoul National University Bundang Hospital) 윤영수 (노원을지대학교병원 성형외과) Kyung Hee Min (Departments of Plastic and Reconstructive Surgery Eulji General Hospital Eulji University School o) 남선영 (Department of Plastic and Reconstructive Surgery Seoul National University Bundang Hospital Seongn) Kyu Sang Lee (2Department of Pathology Seoul National University Bundang Hospital) Byung Ho Shin (Seoul National University College of Medicine) Seung-Hee Lee (Seoul National University College of Medicine) 이미지 (Department of Plastic and Reconstructive Surgery Seoul National University Bundang Hospital Seongn)
저널정보
대한미용성형외과학회 Archives of Aesthetic Plastic Surgery Archives of Aesthetic Plastic Surgery Vol.27 No.3
발행연도
2021.1
수록면
81 - 87 (7page)

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Background Autologous fat grafts are commonly used in cosmetic and reconstructive surgery, and various methods are used to improve their viability. Low-level laser therapy (LLLT) can enhance the proliferation, growth, and differentiation of various cell lines, including stem cells. Our study investigated and compared the effects of LLLT and the addition of adipose-derived stem cells (ADSCs) on the viability of fat grafts. Methods Twenty nude mice were divided into four groups: control (group 1), LLLT irradiation (group 2), ADSC addition (group 3), and LLLT irradiation+ADSC addition (group 4). ADSCs were combined with the fat tissue. LLLT irradiation was performed once daily for 1 week from the day of grafting. After 8 weeks, the weight, volume, histology, and Western blot findings of the grafted fat tissues were evaluated. Results The retention rate and volume of the fat tissue in groups 2, 3, and 4 were higher than that of group 1, but the difference was not statistically significant. The number of capillaries, histological parameters, and immunofluorescence staining analyses for CD68, CD31, fibroblast growth factor, and vascular endothelial growth factor (VEGF) showed no significant differences among the four groups. The expression level of VEGF was higher in group 2 than in the other groups, but not to a statistically significant level. Conclusions LLLT and ADSCs did not significantly improve the viability of autologous fat grafts. Therefore, further study is necessary to develop safe and effective methods to improve the viability of these grafts for clinical application.

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