지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Probiotic-derived bacteriocins have attracted considerable attention as an alternative to synthetic preservatives in the food industry, as synthetics are harmful to health. Lactobacillus gasseri G2 isolated from infant feces has been demonstrated to possess probiotic properties such as bile salt and acid tolerance, antibiotic resistance, and broad antimicrobial activity. Bacteriocin was partially purified by acetone precipitation and characterized for several properties. It was found to be highly thermostable and resistant to heating at 120oC for 15 min. Bacteriocin activity remained unaffected with changes in the pH range from 2.0 to 10.0 and by solvents such as acetone, methanol, ethanol, and butanol. The antimicrobial activity was not affected by α-amylase, lysozyme, and lipase, but was reduced after treatment with protease, trypsin, and proteinase K, suggesting that it has a proteinaceous nature. Bacteriocin inhibited a broad range of target bacteria belonging to both Gram-positive and Gram-negative groups: Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, Escherichia coli O157:H7, Enterobacter sakasakii, Salmonella Enteritidis, Shigella flexneri, Shigella boydii, Vibrio parahaemolyticus, and Vibrio vulnificus. Bacteriocin appeared as a smear of the protein band with a molecular weight between 2-5 kDa, which showed a clear zone of inhibition after bioassay. The high thermostability and acid/alkali stability of bacteriocin reflect its potential commercial significance.
목차
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참고문헌 (8)
참고문헌 신청
Pei J / 2018 / Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood / Lett. Appl. Microbiol 66:38~43
Rahmeh R / 2019 / Distribution and antimicrobial activity of lactic acid bacteria from raw camel milk / New Microbes. New Infect 30:100560
Ruiz-Barba JL / 1994 / Use of Lactobacillus plantarum LPCO10, a bacteriocin producer, as a starter culture in Spanish-style green olive fermentations / Appl. Environ. Microbiol 60:2059~2064
Silva CC / 2018 / Application of bacteriocins and protective cultures in dairy food preservation / Front. Microbial 9:594
Toba T / 1991 / Potential of Lactobacillus gasseri isolated from infant faeces to produce bacteriocin / Lett. Appl. Microbiol 12:228~231
Rahmeh R / 2019 / Distribution and antimicrobial activity of lactic acid bacteria from raw camel milk / New Microbes. New Infect 30:100560
Ruiz-Barba JL / 1994 / Use of Lactobacillus plantarum LPCO10, a bacteriocin producer, as a starter culture in Spanish-style green olive fermentations / Appl. Environ. Microbiol 60:2059~2064
Silva CC / 2018 / Application of bacteriocins and protective cultures in dairy food preservation / Front. Microbial 9:594
Toba T / 1991 / Potential of Lactobacillus gasseri isolated from infant faeces to produce bacteriocin / Lett. Appl. Microbiol 12:228~231
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