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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 270 - 274 (5page)
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Green tea, the leaves of Camellia sinsneis (Theaceae), is generally acknowledged as the most consumed beverage with multiple pharmacological functions including antioxidant activity. This study was performed to analyze the effect of extraction conditions of green tea on its antioxidant effects using DPPH assay. Three extraction factors such as extraction solvent (EtOH, 0 - 100%), extraction time (3 - 15 min) and extraction temperature (10 - 70℃) were analyzed and optimized extraction condition for antioxidant activity of green tea extract (GTE) was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of data and the optimal conditions of extraction were found to be 57.7% EtOH, 15 min and 70℃. Under this condition, antioxidant activity of experimental data was 88.4% which was almost fit to the ideal value of 88.6%. As epigallocatechin gallate (EGCG) is known for the major ingredient for antioxidant activity of green tea, we investigated the effect of EGCG on antioxidant activity of GTE. EGCG showed antioxidant activity with the IC<sub>50</sub> value of 4.2 μg/ml and a positive correlation was observed between EGCG content and the antioxidant activity of GTE with R² = 0.7134. Interestingly, however, GTE with 50 - 70% antioxidant activity contain less than 1.0 μg/ml of EGCG, which is much lower than IC<sub>50</sub> value of EGCG. Therefore, we suppose that EGCG together with other constituents contribute to antioxidant activity of GTE. Taken together, these results suggest that green tea is more beneficial than EGCG alone for antioxidant ability and optimal extraction condition of green tea will be useful for the development of food and pharmaceutical applications
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참고문헌 (23)
참고문헌 신청
Zhang, L. / 2011 / Proteomics 11:4638~4647
Julie Bornhoeft / 2012 / The Protective Effects of Green Tea Polyphenols: Lipid Profile, Inflammation, and Antioxidant Capacity in Rats Fed an Atherogenic Diet and Dextran Sodium Sulfate / Journal of Medicinal Food 15(8):726~732
Rickman, C. / 2010 / Cur. Pharm. Biotechnol 11:881~886
Ramadan, G. / 2009 / Br. J. Nutr 102:1611~1619
Du, G. J. / 2012 / Nutrients 4:1679~1691
Julie Bornhoeft / 2012 / The Protective Effects of Green Tea Polyphenols: Lipid Profile, Inflammation, and Antioxidant Capacity in Rats Fed an Atherogenic Diet and Dextran Sodium Sulfate / Journal of Medicinal Food 15(8):726~732
Rickman, C. / 2010 / Cur. Pharm. Biotechnol 11:881~886
Ramadan, G. / 2009 / Br. J. Nutr 102:1611~1619
Du, G. J. / 2012 / Nutrients 4:1679~1691
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