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논문 기본 정보

자료유형
학술저널
저자정보
Kang Yun Ji (Division of Applied Life Science (BK21 Four) Graduate School Gyeongsang National University Jinju 5) Kim Tae Jin (Division of Applied Life Science (BK21 Four) Graduate School Gyeongsang National University Jinju 5) Kim Min Jae (Division of Applied Life Science (BK21 Four) Graduate School Gyeongsang National University Jinju 5) Yoo Ji Yeon (Division of Applied Life Science (BK21 Four) Graduate School Gyeongsang National University Jinju 5) Kim Jeong Hwan (Division of Applied Life Science (BK21 Four) Graduate School Gyeongsang National University Jinju 5)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제51권 제2호
발행연도
2023.6
수록면
157 - 166 (10page)
DOI
10.48022/mbl.2305.05004

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초록· 키워드

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Three lactic acid bacteria (LAB) producing exopolysaccharides (EPSs) were isolated from Pa (green onion)- kimchi, and identified as Weissella confusa (SKP 173), Weissella cibaria (SKP 182), and Leuconostoc citreum (SKP 281), respectively by 16S rRNA gene sequencing. The yields of EPS were 21.27, 18.53, and 15.4 g/l for EPS from SKP 173, 182, and 281, respectively when grown in MRS broth containing sucrose (5%, w/v). Total sugar contents were 64.39, 62.84, and 65.16% (w/w) for EPS from SKP 173, 182, and 281, respectively while the protein contents were 0.33, 0.31, and 0.25% (w/w), respectively. EPSs from W. confusa SKP 173 and W. cibaria SKP 182 contained glucose only but EPS from L. citreum SKP 281 contained glucose and glucitol. Viscosities of the 2% (w/w) freeze-dried EPS solution were 9.60, 8.00, and 8.20 centipoise (cP) for EPS from SKP 173, 182, and 281, respectively. Viscosities of culture grown in MRS broth with 5% sucrose (no glucose) were 92.98, 57.19, and 18.8 cP, respectively. The average molecular weights of EPSs were larger than 2 × 107 Da. Fourier transform infrared spectroscopy (FT-IR) analyses of EPSs showed typical carbohydrate peaks, suggesting that the EPSs consisted of pyranose saccharides with α-(1,6) and α-(1,3) glycosidic linkages. L. citreim SKP 281 was used as the starter for yogurt fermentation, and EPS production was confirmed.

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