지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.1
- 수록면
- 580 - 587 (8page)
이용수
초록· 키워드
오류제보하기
The serine protease urokinase-type plasminogen
activator (uPA) is im plicated in pericellular proteolysis
in a variety of physiological and pathological
processes including angiogenesis and tumor
metastasis. The kringle domain of uPA (UK1) has
proven to be an anti-angiogenic molecule with unknown
mechanism and amino terminal fragment of
uPA (u-ATF) with additional growth factor-like domain
can be used for blocking interaction of uPA
and uPA receptor. Here, we compared anti-angiogenic
activities of these two molecules in vitro and
in vivo. The recombinant u-ATF from E. coli and
refolded in vitro was found to bind to uPAR with
high affinity, whereas E. coli-derived UK1 showed
no binding by Biacore analysis. In contrast to UK1
having potent inhibitory effect, u-ATF exhibited low
inhibitory effect on bovine capillary endothelial
cell growth (ED50＞320 nM). Furthermore, u-ATF
inhibition of VEGF-induced migration of human
umbilical vein endothelial cell was far less sensitive
(IC50 = 600 nM) than those observed with UK1,
and angiogenesis inhibition was marginal in chorioallantoic
membrane. These results suggest that
kringle domain alone is sufficient for potent antiangiogenic
activity and additional growth factor-like
domain diverts this molecule in undergoing different
mechanism such as inhibition of uPA/uPAR
interaction rather than undergoing distinct antiangiogenic
mechanism driven by kringle domain.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Expression, Purification, and Biological Characterization of The Amino-Terminal Fragment of Urokinase in Pichia pastoris
Journal of Microbiology and Biotechnology
2013 .01
-
Plasminogen kringle 5 재조합 단백질에 의한 ERK1/2 활성화 및 세포골격 재배열 억제
생명과학회지
2006 .12
-
Regulation of hepatocyte growth factor-mediated urokinase plasminogen activator secretion by MEK/ERK activation in human stomach cancer cell lines
Experimental and Molecular Medicine
2006 .01
-
Reactive oxygen species regulate the generation of urokinase plasminogen activator in human hepatoma cells via MAPK pathways after treatment with hepatocyte growth factor
Experimental and Molecular Medicine
2009 .01
-
Anti-Angiogenic Cancer Gene Therapy with Apolipoprotein (a) Kringle Genes
한국미생물학회 학술대회논문집
2006 .05
-
ATF2 전사인자의 발현과 AP-1 전사인자인 BATF, c-Fos, c-Jun과의 이량체 형성
생명과학회지
2005 .12
-
Development of a Chimera Molecule, GFP-ATF6 to monitor Stress-Condition of Cells in vitro
한국생명과학회 심포지움
2006 .04
-
SIRT1 Suppresses Activating Transcription Factor 4 (ATF4) Expression in Response to Proteasome Inhibition
Journal of Microbiology and Biotechnology
2013 .01
-
T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhancesanti-angiogenic and anti-tumor activity via αvβ3 and α5β1integrins
Experimental and Molecular Medicine
2008 .01
-
Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB
Experimental and Molecular Medicine
2011 .01
-
A serum-stable branched dimeric anti-VEGF peptide blocks tumor growth via anti-angiogenic activity
Experimental and Molecular Medicine
2010 .01
-
Curcumin Inhibits Cell Proliferation of Human Colorectal HCT116 Cells through Up-Regulation of Activating Transcription Factor 3 (ATF3)
생명과학회지
2012 .04
-
Urokinase Inhibitor Design Based on Pharmacophore Model Derived from Diverse Classes of Inhibitors
Bioinformatics and Biosystems
2006 .01
-
Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
Experimental and Molecular Medicine
2016 .01
-
Involvement of MAPK pathway in hypoxia-induced up-regulation of urokinase plasminogen activator receptor in a human prostatic cancer cell line, PC3MLN4
Experimental and Molecular Medicine
2004 .01
-
Rhus verniciflua Stokes extract suppresses migration and invasion in human gastric adenocarcinoma AGS cells
Nutrition Research and Practice
2020 .10
-
Rhus verniciflua Stokes extract suppresses migration and invasion in human gastric adenocarcinoma AGS cells
Nutrition Research and Practice
2020 .10
-
KR-31831, a new synthetic anti-ischemic agent, inhibits in vivo and in vitro angiogenesis
Experimental and Molecular Medicine
2006 .01
-
올리고 마이크로어래이를 이용한 활성화된 인간 제대 정맥 내피세포의 유전자 발현 조사
생명과학회지
2004 .10
-
Development of a Reporter System Monitoring Regulated Intramembrane Proteolysis of the Transmembrane bZIP Transcription Factor ATF6α
Molecules and Cells
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0