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자료유형
학술저널
저자정보
저널정보
한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.3 No.4
발행연도
1998.12
수록면
329 - 333 (5page)

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Cell wall(lactic acid bacteria-sonicated precipitate: LAB-SP) and cytosol (lactic acid bacteria-sonicated supernatant: LAB-SS) fractions were prepared from kimchi fermenting lactic acid bacteria such as Leuconostoc mesenteroides, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus plantarum and Pediococcus acidilactici, with Lactobacillus acidophillus isolated from yogurt. Using the Ames mutagenicity test and SOS chromotest system, the antimutagenic activity of those cell fractions was studied. One hundred eighty μl of LAB-SP from lactic acid bacteria isolated from kimchi, excepting Pediococcus acidilactici, supressed the mutagenicity of 4-nitroquinoline-1-oxide (4-NQO) in Ames mutagenicity test and SOS chromotest system, by above 90% and 60%, respectively. LAB-SP from lactic acid bacteria exhibited the antimutagenic activity aganist 2-amino-3,4-dimethyl-imidazo (4,5-f) quinoline (MeIQ) in Ames mutagenicity test, depending on the concentration. Especially, Lactobacillus plantarum which were isolated from kimchi had the strongest antimutagenicity on MeIQ. LAB-SP from lactic acid bacteria also inhibited the mutagenicity mediated by 3-amino-1-methyl-5H-pyrido [4,3-b]indole (Trp-P-2). Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus acidphillus had higher antimutagenicity against Trp-P-2 than the other lactic acid bacteria. However, LAB-SS of lactic acid bacteria did not show any mutagenic activity against 4-NQO in Ames mutagenicity test and SOS chromotest systems. On the mutagenicity of MeIQ and Trp-P-2, LAB-SS of lactic acid bacteria from kimchi or dairy products exhibited a weaker inhibitory effect than LAB-SP of those bacteria. These results represent that, whether the lactic acid bacteria from kimchi are viable or nonviable, antimutagenic activity was still effective. We suggest that the strong, antimutaganic activity of lactic acid bacteria might be found in the cell wall fraction, rather than in the cytosol fraction.

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Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

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