지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 9 - 15 (7page)
이용수
초록· 키워드
오류제보하기
Receptor activator of nuclear factor-κB ligand (RANKL) is an osteoblast/stromal cell-derived essential factor for osteoclastogenesis. During endochondral bone formation, hypertrophic chondrocytes calcify cartilage matrix that is subsequently resorbed by osteoclasts in order to be replaced by new bone. Hypoxia-induced upregulation of RANKL expression has been previously demonstrated in an in vitro system using osteoblasts; however, the involved mechanism remains unclear in chondrocytes. In the present study, we investigated whether hypoxia regulates RANKL expression in ATDC5 cells, a murine chondrogenic cell line, and hypoxiainducible factor-1α (HIF-1α) mediates hypoxia-induced RANKL expression by transactivating the RANKL promoter. The expression levels of RANKL mRNA and protein, as well as HIF-1α protein, were significantly increased in ATDC5 cells under hypoxic condition. Constitutively active HIF-1α alone significantly increased the levels of RANKL expression under normoxic conditions, whereas dominant negative HIF-1α reduced hypoxia-induced RANKL expression. HIF-1α increased RANKL promoter reporter activity in a HIF-1α binding element-dependent manner in ATDC5 cells. Hypoxia-induced RANKL levels were much higher in differentiated ATDC5 cells, as compared to proliferating ATDC5 cells. These results suggested that under hypoxic conditions, HIF-1α mediates induction of RANKL expression in chondrocytes; in addition, hypoxia plays a role in osteoclastogenesis during endochondral bone formation, at least in part, through the induction of RANKL expression in hypertrophic chondrocytes.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
B. C. J. van der Eerden / 2003 / Systemic and Local Regulation of the Growth Plate / Endocrine Reviews 24(6):782~801
Michihiko Usui / 2008 / Murine and Chicken Chondrocytes Regulate Osteoclastogenesis by Producing RANKL in Response to BMP2 / Journal of Bone and Mineral Research 23(3):314~325
Hisahiro Yoshida / 1990 / The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene / Nature 345(6274):442~444
Kenichiro Matsuzaki / 1998 / Osteoclast Differentiation Factor (ODF) Induces Osteoclast-like Cell Formation in Human Peripheral Blood Mononuclear Cell Cultures / Biochemical and Biophysical Research Communications 246(1):199~204
D.L Lacey / 1998 / Osteoprotegerin Ligand Is a Cytokine that Regulates Osteoclast Differentiation and Activation / Cell 93(2):165~176
Michihiko Usui / 2008 / Murine and Chicken Chondrocytes Regulate Osteoclastogenesis by Producing RANKL in Response to BMP2 / Journal of Bone and Mineral Research 23(3):314~325
Hisahiro Yoshida / 1990 / The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene / Nature 345(6274):442~444
Kenichiro Matsuzaki / 1998 / Osteoclast Differentiation Factor (ODF) Induces Osteoclast-like Cell Formation in Human Peripheral Blood Mononuclear Cell Cultures / Biochemical and Biophysical Research Communications 246(1):199~204
D.L Lacey / 1998 / Osteoprotegerin Ligand Is a Cytokine that Regulates Osteoclast Differentiation and Activation / Cell 93(2):165~176
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Hypoxia Inducible Factor-1α Directly Induces the Expression of Receptor Activator of Nuclear Factor-κB Ligand in MLO-Y4 Osteocytes
International Journal of Oral Biology
2015 .01
-
Development of Inhibitors Targeting Hypoxia-Inducible Factor 1 and 2 for Cancer Therapy
Yonsei Medical Journal
2017 .01
-
Hypoxia Induces Epithelial-Mesenchymal Transition in Follicular Thyroid Cancer: Involvement of Regulation of Twist by Hypoxia Inducible Factor-1α
Yonsei Medical Journal
2015 .01
-
Is Biochemical Follow Up Possible in Peripheral Arterial Disease Treatment: Hypoxia Inducible Factor-1 Alpha?
Vascular Specialist International
2021 .09
-
Hypoxia-Induced Suppression of Antiapoptotic Bcl-2 Expression in Human Bladder Tumor Cells Is Regulated by Caveolin-1-Dependent Adenosine Monophosphate-Activated Protein Kinase Activity
International Neurourology Journal
2021 .01
-
Hypoxia-Inducible Factor-1 Alpha Stabilization in Human Macrophages during Leishmania major Infection Is Impaired by Parasite Virulence
Parasites, Hosts and Diseases
2022 .10
-
P2Y<sub>6</sub> 수용체 길항제의 파골세포 분화 촉진 효과 규명
약학회지
2015 .01
-
Hypoxia-Induced Suppression of Antiapoptotic Bcl-2 Expression in Human Bladder Tumor Cells Is Regulated by Caveolin-1-Dependent Adenosine Monophosphate-Activated Protein Kinase Activity
International Neurourology Journal
2021 .06
-
Genipin inhibits hypoxia-induced accumulation of HIF-1α and VEGF expression in human cervical carcinoma HeLa cells
고신대학교 의과대학 학술지
2019 .01
-
저산소 조건에 의한 HepG2 세포주의 성장에서 HIF-1α 비의존적 기전의 확인
Journal of Liver Cancer
2016 .01
-
ACY-241, a histone deacetylase 6 inhibitor, suppresses the epithelial–mesenchymal transition in lung cancer cells by downregulating hypoxia-inducible factor-1 alpha
The Korean Journal of Physiology & Pharmacology
2024 .01
-
Attenuation of RANKL-induced Osteoclast Formation via p38-mediated NFATc1 Signaling Pathways by Extract of Euphorbia Lathyris L
대한골대사학회지
2016 .01
-
Overexpression of CD44 Standard Isoform Upregulates HIF-1α Signaling in Hypoxic Breast Cancer Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2018 .01
-
Controlled Gene Expression System under Hypoxia Conditions
항공우주의학 학술대회
2015 .05
-
Isoliquiritigenin Inhibits Metastatic Breast Cancer Cell-induced Receptor Activator of Nuclear Factor Kappa-B Ligand/Osteoprotegerin Ratio in Human Osteoblastic Cells
대한암예방학회지
2015 .01
-
MSK1 regulates RANKL-induced NFATc1 expression through CREB and c-Fos
Journal of Biomedical and Translational Research
2015 .01
-
녹제초 추출물이 파골세포 분화 및 골 흡수에 미치는 영향
한방재활의학과학회지
2019 .01
-
HIF2α/EFEMP1 cascade mediates the effects of hypoxia on breast cancer stem cell hierarchy
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Effect of low-level laser therapy on bisphosphonate-treated osteoblasts
Maxillofacial Plastic Reconstructive Surgery
2016 .11
-
꾸지뽕나무 뿌리 추출물의 파골세포 분화 억제 효과
생약학회지(Korean Journal of Pharmacognosy)
2017 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0