지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 615 - 621 (7page)
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이 논문의 연구 히스토리 (2)
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Background and Objectives Polyinosinic-polycytidylic acid (Poly I:C) is structurally similar to double-stranded RNA, and is known to induce various inflammatory mediators and to cause inflammatory reactions in airway epithelial cells. However, the effect of Poly I:C on secretion of mucins in human airway epithelial cells has been very rarely reported. In this study, the effect and brief signaling pathway of Poly I:C on the expression of mucin genes were investigated in human airway epithelial cells.
Materials and Method In mucin-producing human NCI-H292 airway epithelial cells and the primary cultures of normal human nasal epithelial cells, the effect and signaling pathway of Poly I:C on expression of mucin genes were investigated using reverse transcriptase-polymerase chain reaction, real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA (siRNA) for mitogen-activated protein kinase (MAPK).
Results Poly I:C induced the MUC5B expression, and activated the phosphorylation of ERK1/2 and p38 MAPK. U0126 (ERK1/2 MAPK inhibitor) and SB203580 (p38 MAPK inhibitor) inhibited the Poly I:C-induced MUC5B expression. In addition, the knockdown of ERK2 and p38 MAPK by siRNA significantly blocked the Poly I:C-induced MUC5B mRNA expression.
Conclusion Poly I:C induces the MUC5B expression via ERK2 and p38 MAPK signaling pathways in human airway epithelial cells. Therefore, Poly I:C may play a role in the regulation of mucus hypersecretion through MAPK signaling pathways in the human airway epithelial cells.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Ali MS / 2007 / Upper airway mucin gene expression: a review / Laryngoscope 117 (5) : 932 ~ 938
Bae CH / 2013 / Insulin-like growth factor-1 induces MUC8 and MUC5B expression via ERK1and p38 MAPK in human airway epithelial cells / Biochem Biophys Res Commun 430 (2) : 6
Hewson CA / 2005 / Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells / J Virol 79 (19) : 122
Kimura K / 2011 / Inhibition of poly(I:C)-induced matrix metalloproteinase expression in human corneal fibroblasts by triptolide / Mol Vis 17 : 526 ~ 532
Kumar H / 2008 / Cutting edge:cooperation of IPS-1- and TRIF-dependent pathways in poly ICenhanced antibody production and cytotoxic T cell responses / J Immunol 80 (2) : 68
Bae CH / 2013 / Insulin-like growth factor-1 induces MUC8 and MUC5B expression via ERK1and p38 MAPK in human airway epithelial cells / Biochem Biophys Res Commun 430 (2) : 6
Hewson CA / 2005 / Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells / J Virol 79 (19) : 122
Kimura K / 2011 / Inhibition of poly(I:C)-induced matrix metalloproteinase expression in human corneal fibroblasts by triptolide / Mol Vis 17 : 526 ~ 532
Kumar H / 2008 / Cutting edge:cooperation of IPS-1- and TRIF-dependent pathways in poly ICenhanced antibody production and cytotoxic T cell responses / J Immunol 80 (2) : 68
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