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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 1,036 - 1,043 (8page)
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Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage,and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type‒inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type‒inducibleluciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and in vivo. Results: NSCs introduced via the neuronal cell type‒inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly,the luciferase expression level of NSE::Luci-NSCs increased greatly afterdifferentiation into neurons. Conclusion: We demonstrated that a neuronal cell type‒inducible gene expression system is suitable for introducing NSCs in combinedtreatment strategies. We suggest that the proposed strategy may be a promisingtool for the treatment of neurodegenerative disorders, including SCI.
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참고문헌 (14)
참고문헌 신청
An SS / 2010 / Neuroprotective effect of combined hypoxia-induced VEGF and bone marrow-derived mesenchymal stem cell treatment / Childs Nerv Syst 26 : 323 ~ 331
Hagg T / 2006 / Degenerative and spontaneous regenerative processes after spinal cord injury / J Neurotrauma 23 : 264 ~ 280
Iwai H / 2014 / Transplantation of neural stem/progenitor cells at different locations in mice with spinal cord injury / Cell Transplant 23 : 1451 ~ 1464
Jin H / 2009 / Role of the oxygen-dependent degradation domain in a hypoxia-inducible gene expression system in vascular endothelial growth factor gene therapy / Spine (Phila
Kim HJ / 2012 / Hypoxia-specific GM-CSF-overexpressing neural stem cells improve graft survival and functional recovery in spinal cord injury / Gene Ther 19 : 513 ~ 521
Hagg T / 2006 / Degenerative and spontaneous regenerative processes after spinal cord injury / J Neurotrauma 23 : 264 ~ 280
Iwai H / 2014 / Transplantation of neural stem/progenitor cells at different locations in mice with spinal cord injury / Cell Transplant 23 : 1451 ~ 1464
Jin H / 2009 / Role of the oxygen-dependent degradation domain in a hypoxia-inducible gene expression system in vascular endothelial growth factor gene therapy / Spine (Phila
Kim HJ / 2012 / Hypoxia-specific GM-CSF-overexpressing neural stem cells improve graft survival and functional recovery in spinal cord injury / Gene Ther 19 : 513 ~ 521
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