지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.6
- 수록면
- 94 - 102 (9page)
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초록· 키워드
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Cereblon (CRBN), a negative modulator of AMP-activated protein kinase (AMPK), is highly expressed in the retina. We confirmed the expression of CRBN in ARPE-19 human retinal cells by Western blotting. We also demonstrated that CRBN knock-down (KD) could effectively downregulate IL-6 and MCP-1 protein and gene expression in LPS-stimulated ARPE-19 cells. Additionally, CRBN KD increased the phosphorylation of AMPK/acetylcoenzyme A carboxylase (ACC) and the expression of heme oxygenase-1 (HO-1) in ARPE-19 cells. Furthermore, CRBN KD significantly reduced LPS-induced nuclear translocation of NF-κB p65 and activation of NF-κB promoter activity. However, these processes could be inactivated by compound C (inhibitor of AMPK) and zinc protoporphyrin-1 (ZnPP-1; inhibitor of HO-1). In conclusion, compound C and ZnPP-1 can rescue LPS-induced levels of proinflammatory cytokines (IL-6 and MCP-1) in CRBN KD ARPE-19 cells. Our data demonstrate that CRBN deficiency negatively regulates proinflammatory cytokines via the activation of AMPK/HO-1 in the retina.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (21)
참고문헌 신청
Wang AL / 2019 / Retinitis pigmentosa : review of current treatment / Int Ophthalmol Clin 59:263~280
Carreño E / 2017 / Cytokine and chemokine tear levels in patients with uveitis / Acta Ophthalmol 95:e405
Pollreisz A / 2012 / Klebsiella pneumoniae induces an inflammatory response in human retinal-pigmented epithelial cells / Biochem Biophys Res Commun 418:33~37
Tode J / 2017 / Intravitreal injection of anti-interleukin(IL)-6 antibody attenuates experimental autoimmune uveitis in mice / Cytokine 96:8~15
Higgins JJ / 2004 / A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation / Neurology 63:1927~1931
Carreño E / 2017 / Cytokine and chemokine tear levels in patients with uveitis / Acta Ophthalmol 95:e405
Pollreisz A / 2012 / Klebsiella pneumoniae induces an inflammatory response in human retinal-pigmented epithelial cells / Biochem Biophys Res Commun 418:33~37
Tode J / 2017 / Intravitreal injection of anti-interleukin(IL)-6 antibody attenuates experimental autoimmune uveitis in mice / Cytokine 96:8~15
Higgins JJ / 2004 / A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation / Neurology 63:1927~1931
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UCI(KEPA) : I410-ECN-0101-2020-517-000894324