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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 723 - 728 (6page)
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A protein binding microarray (PBM) analysis was performed to determine the nucleotide sequence responsible for the specific binding of FUS3, a transcription factor in Arabidopsis that is considered to be a master regulator of seed development. Full-length FUS3 cDNA was fused in-frame with sequences encoding the fluorescent protein DsRed and a polyhistidine tag, and expressed in Escherichia coli. The partially purified fusion protein was incubated with the quadruple 9-mer PBM. An analysis of fluorescent images revealed that the fusion protein recognized and bound to the consensus sequence CATGCA. This result suggests that, among the nucleotides contained within the RY motif (CATGCA[TG]), six nucleotides (CATGCA) are sufficient for FUS3-specific binding. It appears that an additional 'TG' at the 3' end of the RY motif found in seed-specific gene promoters was not necessary for binding specificity. Notably, FUS3 may also bind to the palindromic RY motif in its dimeric form.
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