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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 601 - 609 (9page)
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Bifidobacterium longum KACC 91563 secretes family 5 extracellular solute-binding protein via extracellular vesicle. In our previous work, it was demonstrated that the protein effectively alleviated food allergy symptoms via mast cell specific apoptosis, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, we cloned the gene encoding extracellular solute-binding protein of the strain into the histidine-tagged pET-28a(+) vector and transformed the resulting plasmid into the Escherichia coli strain BL21 (DE3). The histidine-tagged extracellular solute-binding protein expressed in the transformed cells was purified using Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the process, two liters of transformed culture produced 7.15 mg of the recombinant proteins. This is the first study describing the production of extracellular solute-binding protein of probiotic bacteria. Establishment of large-scale production strategy for the protein will further contribute to the development of functional foods and potential alternative treatments for allergies.
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참고문헌 (22)
참고문헌 신청
Aguilar-Toala JE / 2018 / Postbiotics : An evolving term within the functional foods field / Trends Food Sci Technol 75:105~114
Ahmad Rather I / 2013 / Isolation and characterization of a proteinaceous antifungal compound from Lactobacillus plantarum YLM007 and its application as a food preservative / Lett Appl Microbiol 57:69~76
Bitto NJ / 2017 / The therapeutic benefit of bacterial membrane vesicles / Int J Mol Sci 18:1287
Chen H / 2003 / Bacteriocins and their food applications / Compre Rev Food Sci Food Saf 2:82~100
El Andaloussi S / 2013 / Extracellular vesicles : Biology and emerging therapeutic opportunities / Nat Rev Drug Discov 12:347~357
Ahmad Rather I / 2013 / Isolation and characterization of a proteinaceous antifungal compound from Lactobacillus plantarum YLM007 and its application as a food preservative / Lett Appl Microbiol 57:69~76
Bitto NJ / 2017 / The therapeutic benefit of bacterial membrane vesicles / Int J Mol Sci 18:1287
Chen H / 2003 / Bacteriocins and their food applications / Compre Rev Food Sci Food Saf 2:82~100
El Andaloussi S / 2013 / Extracellular vesicles : Biology and emerging therapeutic opportunities / Nat Rev Drug Discov 12:347~357
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