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자료유형
학술저널
저자정보
Amjadi, Fatemehsadat (Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences) Zandieh, Zahra (Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences) Salehi, Ensieh (Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR) Jafari, Reza (Department of Immunology, Iran University of Medical Sciences) Ghasemi, Nasrin (Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences) Aflatoonian, Abbas (Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences) Fazeli, Alireza (Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield) Aflatoonian, Reza (Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제45권 제1호
발행연도
2018.1
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Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1-10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands. Results: Fallopian tube epithelial cells expressed TLRs 1-10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8. Conclusion: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos.

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