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논문 기본 정보

자료유형
학술저널
저자정보
Jung, Tae-Hwan (Department of Health and Bio-Convergence, Sahmyook University) Choi, Jae-Hwan (Department of Food Science and Biotechnology, Sahmyook University) Koh, Kyung-Chul (Korea Meat Research Institute) Jeon, Woo-Min (Department of Animal Biotechnology and Resource, Sahmyook University) Han, Kyoung-Sik (Department of Health and Bio-Convergence, Sahmyook University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제37권 제5호
발행연도
2017.1
수록면
743 - 751 (9page)

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During slaughtering, animal blood is typically discarded, resulting in water pollution. However, this discarded blood has valuable components, such as immunoglobulin (Ig). Although several studies have been conducted to develop methods for effective recycling of slaughterhouse blood, they have not been commercially utilized in Korea. Here, we extracted an Ig-rich fraction from porcine blood that was then subjected to various in vitro tests, including pathogen growth inhibition, antigenic cross-reactivity, and anti-toxin activity. The porcine immunoglobulin concentrate (PIC) was effectively purified by eliminating other components, such as albumin, and consisted of approximately $63.2{\pm}2.9%$ IgG and $7.2{\pm}0.4%$ IgM on a protein basis. The results showed that it significantly suppressed the growth of pathogenic bacteria, and bound to all tested pathogens, including both gram-positive and gram-negative species, although the degree of activity differed according to strain. The PIC bound to two types of lipopolysaccharide (LPS) obtained from Escherichia coli O111:B4 and Salmonella enterica serotype typhimurium in a concentration-dependent manner. In addition, the PIC restored the proliferation activity of the lymphoblast K-562 cells when co-incubated with pathogenic LPS. These results confirm that the PIC prepared in this study is a potentially valuable functional food material or diet supplement as an alternative to antibiotics that can protect animals from pathogenic bacteria.

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