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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 저널정보
- 한국수산과학회 양식분과 한국수산과학회 양식분과 학술대회 2021 Conference on the Future Technology of Fisheries Science [3개 분과 공동개최]
- 발행연도
- 2021.4
- 수록면
- 185 - 185 (1page)
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초록· 키워드
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Human norovirus (HNoV) is the most common cause of gastroenteritis worldwide. Manila clam (Ruditapes philippinarum), a species belonging to the Veneridae of the bivalve is considered as a relatively high risk food for HNoV. In food processing, hypochlorite (NaOCl) is used as the most common disinfectant for a wide range of harmful microorganisms including pathogens. HuNoV contaminated food and water can be detected by real-time quantitative-PCR (RT-qPCR). However, this PCR approach can be used to analyze HNoV survival because it detect both non-infectious and infectious cells of the virus. Propidium monoazide (PMA) as the nucleic acid-intercalating agent can be applied as a pretreatment prior to RT-qPCR to distinguish between live and dead cells. Therefore, this study investigated the effect of NaOCl was evaluated for inactivation of HNoV GII.4, a dominant genotype in manila clams treated by NaOCl solution at 100, 300, 500, 700, and 1,000 ppm of Cl<sub>2</sub> for 10 min using a RT-qPCR combined with PMA. HNoV GII.4 were significantly (p<0.05) reduced to 0.37.0.58, 0.85, 0.99, and 1.54 log<sub>10</sub>copy number/mL by 100 ... 전체 초록 보기
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UCI(KEPA) : I410-ECN-0101-2021-529-001944721