Comparative gene expression profiling reveals key pathways and genes different in skin epidermal stem cells and corneal epithelial cells

Yanjie Guo; Weini Wu; Xiya Ma; Mingyan Shi; Xueyi Yang

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자료유형: 학술저널

저자정보: Yanjie Guo (Luoyang Normal University) Weini Wu (Luoyang Normal University) Xiya Ma (Luoyang Normal University) Mingyan Shi (Luoyang Normal University) Xueyi Yang (Luoyang Normal University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.41 No.6

발행연도: 2019.1

수록면: 679 - 688 (10page)

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Background Corneal epithelial cells (CECs) are required for corneal transparency and visual function, and corneal injuries may cause corneal blindness. Skin epidermal stem cells (SESCs), which share the same origin with CECs and have the potential of multi-directional differentiation are ideal seed cells for tissue engineered corneal construction to treat corneal blindness. Objective This study aims to investigate critical genes and pathways that may modulate the transdifferentiation from SESCs to CECs. Methods Isolated SESCs and CECs were used for gene expression analysis by microarray. GO and KEGG pathway of differently expressed genes (DEGs) were enriched using DAVID. The protein–protein interaction (PPI) network were then constructed using Cytoscape and highly interconnected module was subsequently isolated from the network by Molecular Complex Detection. Expression of the hub genes and other selected genes were then verified by qRT-PCR. Results We found 112 upregulated and 105 downregulated genes in CECs compared with SESCs. These DEGs were significantly enriched in focal adhesion, PI3K–Akt and TNF signaling pathway. Highly interconnected module of PPI network contains ten genes. Further regulatory network of these genes showed that ESR1 and SLC2A4 were hub genes. Conclusion Our study identified gene expression in SESCs and CECs and suggested that several crucial genes and pathways may play critical roles in transdifferentiation from SESCs to CECs. It may help uncover molecular mechanisms and offer a foundation for promoting tissue-engineered cornea into clinical application.

#Corneal epithelia cells · Skin epidermal stem cells · Microarray · Transdifferentiation · Genes expression profiling

참고문헌 (41)

참고문헌 신청

Ahmad S / 2007 / Differentiation of human embryonic stem cells into corneal epithelial-like cells by in vitro replication of the corneal epithelial stem cell niche / Stem Cells 25:1145~1155

Blazejewska EA / 2009 / Corneal limbal microenvironment can induce transdifferentiation of hair follicle stem cells into corneal epithelial-like cells / Stem Cells 27:642~652

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Chen HC / 2012 / Expression of angiogenesis-related factors in human corneas after cultivated oral mucosal epithelial transplantation / Investig Ophthalmol Vis Sci 53:5615~5623

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