지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.12
- 수록면
- 198 - 204 (7page)
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초록· 키워드
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Purpose: Human mesenchymal stem cells (hMSCs) have the potency for self-renewal and differentiation into various kinds of cells. The hMSCs are obtained from the various tissues, including adipose tissue, bone marrow and cord blood. The extracellular matrix (ECM) is an important factor that affects cell adherence, growth, migration, apoptosis and differentiation both in vitro and vivo. The adipose-derived mesenchymal stem cells (AD-MSCs) have CD29 (integrin) on the cell surface, which is the receptor for fibronectin. The aim of this study is to validate the efficacy of ECM, and especially fibronectin, for cell expansion.
Methods: The AD-MSCs were obtained from the abdominal fat of humans. These cells were seeded onto culture plates coated with fibronectin-Human (FN) and plates without ECM (control). The cells were incubated for 3 passages and the cellular morphology was simultaneously observed with microscopy. CCK-8 assay was performed to compare the proliferation ability in each condition at the same passage. Immunocytochemistry staining for integrin-beta1 was performed to observe the cell to cell interaction.
Results: The hAD-MSCs in the FN-coated and non-coated plates exhibited cytoplasm staining for integrin-beta1. In all the cultures, extended fibroblastic-shaped cells that turned into rhomboid cells were most frequently observed. The cell growth rates for the non coated culture plate were lower than those for the FN coated plates. After 72 hour culture under the different coated concentrations of FN and the non coated condition (control), the control group had a lower growth rate. In the culture with a FN coated plate, a significant change was observed as compared with that of the control group. We observed an increase in cell proliferation, with a maximum of 140%, on the FN coated plate by performing CCK-8 assay. In comparison, integrin β1 on the cells was more expressed in the FN-coated plates than that in the non-coated plates.
Conclusion: The cell morphology can be changed faster in the FN coated culture plates than that in the non coated culture plates. Because proliferation and adhesion with FN can enhance the expansion, the culture within a FN coated plate is needed to encourage hAD-MSCs to proliferate in vitro.
Methods: The AD-MSCs were obtained from the abdominal fat of humans. These cells were seeded onto culture plates coated with fibronectin-Human (FN) and plates without ECM (control). The cells were incubated for 3 passages and the cellular morphology was simultaneously observed with microscopy. CCK-8 assay was performed to compare the proliferation ability in each condition at the same passage. Immunocytochemistry staining for integrin-beta1 was performed to observe the cell to cell interaction.
Results: The hAD-MSCs in the FN-coated and non-coated plates exhibited cytoplasm staining for integrin-beta1. In all the cultures, extended fibroblastic-shaped cells that turned into rhomboid cells were most frequently observed. The cell growth rates for the non coated culture plate were lower than those for the FN coated plates. After 72 hour culture under the different coated concentrations of FN and the non coated condition (control), the control group had a lower growth rate. In the culture with a FN coated plate, a significant change was observed as compared with that of the control group. We observed an increase in cell proliferation, with a maximum of 140%, on the FN coated plate by performing CCK-8 assay. In comparison, integrin β1 on the cells was more expressed in the FN-coated plates than that in the non-coated plates.
Conclusion: The cell morphology can be changed faster in the FN coated culture plates than that in the non coated culture plates. Because proliferation and adhesion with FN can enhance the expansion, the culture within a FN coated plate is needed to encourage hAD-MSCs to proliferate in vitro.
목차
서론
방법
결과
고찰
참고문헌
참고문헌 (21)
참고문헌 신청
1. [학술지(정기간행물)] - Pittenger MF / 1999 / Multilineage potential of adult human mesenchymal stem cells / Science 284 : 143 ~ 147
2. [학술지(정기간행물)] - Song GB / 2008 / Growth and proliferation of bone marrow mesenchymal stem cells affected by type I collagen, fibronectin and bFGF / Materials Science & Engineering CB 28 : 1467 ~ 1471
3. [학술지(정기간행물)] - Hutchings H / 2003 / Extracellular matrix-bound vascular endothelial growth factor promotes endothelial cell adhesion, migration, and survival through integrin ligation / FASEB J 17 : 1520 ~ 1522
4. [학술지(정기간행물)] - Goessler UR / 2008 / Integrin expression in stem cells from bone marrow and adipose tissue during chondrogenic differentiation / Int J Mol Med 21 : 271 ~ 279
5. [학술지(정기간행물)] - Hynes RO / 2002 / Integrins: Bidirectional, allosteric signaling machines / Cell 110 : 673 ~ 687
2. [학술지(정기간행물)] - Song GB / 2008 / Growth and proliferation of bone marrow mesenchymal stem cells affected by type I collagen, fibronectin and bFGF / Materials Science & Engineering CB 28 : 1467 ~ 1471
3. [학술지(정기간행물)] - Hutchings H / 2003 / Extracellular matrix-bound vascular endothelial growth factor promotes endothelial cell adhesion, migration, and survival through integrin ligation / FASEB J 17 : 1520 ~ 1522
4. [학술지(정기간행물)] - Goessler UR / 2008 / Integrin expression in stem cells from bone marrow and adipose tissue during chondrogenic differentiation / Int J Mol Med 21 : 271 ~ 279
5. [학술지(정기간행물)] - Hynes RO / 2002 / Integrins: Bidirectional, allosteric signaling machines / Cell 110 : 673 ~ 687
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UCI(KEPA) : I410-ECN-0101-2014-514-001253349