Differential Protein Expressions in Virus-Infected and Uninfected Trichomonas vaginalis

Ding He; Gong Pengtao; Yang Ju; Li Jianhua; Li He; Zhang Guocai; Zhang Xichen

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저자정보: Ding He (Jilin University) Gong Pengtao (Jilin University) Yang Ju (Jilin University) Li Jianhua (Jilin University) Li He (Jilin University) Zhang Guocai (Jilin University) Zhang Xichen (Jilin University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.55 No.2

발행연도: 2017.4

수록면: 121 - 128 (8page)

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Protozoan viruses may influence the function and pathogenicity of the protozoa. Trichomonas vaginalis is a parasitic protozoan that could contain a double stranded RNA (dsRNA) virus, T. vaginalis virus (TVV). However, there are few reports on the properties of the virus. To further determine variations in protein expression of T. vaginalis, we detected 2 strains of T. vaginalis; the virus-infected (V+) and uninfected (V-) isolates to examine differentially expressed proteins upon TVV infection. Using a stable isotope N-terminal labeling strategy (iTRAQ) on soluble fractions to analyze proteomes, we identified 293 proteins, of which 50 were altered in V+ compared with V- isolates. The results showed that the expression of 29 proteins was increased, and 21 proteins decreased in V+ isolates. These differentially expressed proteins can be classified into 4 categories: ribosomal proteins, metabolic enzymes, heat shock proteins, and putative uncharacterized proteins. Quantitative PCR was used to detect 4 metabolic processes proteins: glycogen phosphorylase, malate dehydrogenase, triosephosphate isomerase, and glucose-6-phosphate isomerase, which were differentially expressed in V+ and V- isolates. Our findings suggest that mRNA levels of these genes were consistent with protein expression levels. This study was the first which analyzed protein expression variations upon TVV infection. These observations will provide a basis for future studies concerning the possible roles of these proteins in host-parasite interactions.

#Trichomonas vaginalis #Trichomonas vaginalis virus #iTRAQ #quantitative PCR #protein expression

참고문헌 (32)

참고문헌 신청

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