지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 103 - 112 (10page)
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초록· 키워드
오류제보하기
Background: 20(S)-Protopanaxadiol 20-O-D-glucopyranoside, also called compound K (CK), exerts antidiabetic effects that are mediated by insulin secretion through adenosine triphosphate (ATP)-sensitive potassium (K<SUB>ATP</SUB>) channels in pancreatic β-cells. However, the antidiabetic effects of CK may be limited because of its low bioavailability.
Methods: In this study, we aimed to enhance the antidiabetic activity and lower the toxicity of CK by including it with β-cyclodextrin (CD) (CD-CK), and to determine whether the CD-CK compound enhanced pancreatic islet recovery, compared to CK alone, in an alloxan-induced diabetic zebrafish model. Furthermore, we confirmed the toxicity of CD-CK relative to CK alone by morphological changes, mitochondrial damage, and TdT-UTP nick end labeling (TUNEL) assays, and determined the ratio between the toxic and therapeutic dose for both compounds to verify the relative safety of CK and CD-CK.
Results: The CD-CK conjugate (EC<SUB>50</SUB> = 2.158µM) enhanced the recovery of pancreatic islets, compared to
CK alone (EC<SUB>50</SUB> = 7.221µM), as assessed in alloxan-induced diabetic zebrafish larvae. In addition, CD-CK (LC<SUB>50</SUB> = 20.68µM) was less toxic than CK alone (LC<SUB>50</SUB> = 14.24µM). The therapeutic index of CK and CD-CK was 1.98 and 9.58, respectively.
Conclusion: The CD-CK inclusion complex enhanced the recovery of damaged pancreatic islets in diabetic zebrafish. The CD-CK inclusion complex has potential as an effective antidiabetic efficacy with lower toxicity.
Methods: In this study, we aimed to enhance the antidiabetic activity and lower the toxicity of CK by including it with β-cyclodextrin (CD) (CD-CK), and to determine whether the CD-CK compound enhanced pancreatic islet recovery, compared to CK alone, in an alloxan-induced diabetic zebrafish model. Furthermore, we confirmed the toxicity of CD-CK relative to CK alone by morphological changes, mitochondrial damage, and TdT-UTP nick end labeling (TUNEL) assays, and determined the ratio between the toxic and therapeutic dose for both compounds to verify the relative safety of CK and CD-CK.
Results: The CD-CK conjugate (EC<SUB>50</SUB> = 2.158µM) enhanced the recovery of pancreatic islets, compared to
CK alone (EC<SUB>50</SUB> = 7.221µM), as assessed in alloxan-induced diabetic zebrafish larvae. In addition, CD-CK (LC<SUB>50</SUB> = 20.68µM) was less toxic than CK alone (LC<SUB>50</SUB> = 14.24µM). The therapeutic index of CK and CD-CK was 1.98 and 9.58, respectively.
Conclusion: The CD-CK inclusion complex enhanced the recovery of damaged pancreatic islets in diabetic zebrafish. The CD-CK inclusion complex has potential as an effective antidiabetic efficacy with lower toxicity.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
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UCI(KEPA) : I410-ECN-0101-2018-524-001598749