The effect of mesenchymal stem cell use on intraabdominal adhesions in a rat model

Gökhan Karaca; Faruk Pehlivanli; Oktay Aydin; Canan Altunkaya; Hafize Uzun; Mehmet Niyaz; Hüseyin Özden; Huri Bulut

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자료유형: 학술저널

저자정보: Gökhan Karaca (Kırıkkale University) Faruk Pehlivanli (Kırıkkale University) Oktay Aydin (Kırıkkale University) Canan Altunkaya (Kırıkkale University) Hafize Uzun (Istanbul University) Mehmet Niyaz (Hacettepe University) Hüseyin Özden (Alaca State Hospital) Huri Bulut (Bezmialem Vakif University)

저널정보: 대한외과학회 Annals of Surgical Treatment and Research Annals of Surgical Treatment and Research Vol.94 No.2

발행연도: 2018.2

수록면: 57 - 62 (6page)

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Purpose: Intra-abdominal adhesions (IAA) are among the most frequently seen pathologies in general surgery practice with an increased morbidity and mortality. In the present study, we investigated the effect of locally applied mesenchymal stem cells (MSCs) on IAA.
Methods: Twenty-four Wistar Albino rats were used in the study. The rats were divided into three groups including: Sham, control, and MSCs group. On day 0, cecum was reached under anesthesia in all groups, except the Sham group. Scraping with a sponge was performed until petechial bleeding occurred. The control group received no treatment. In the stem cell group, MSCs were applied topically immediately after surgery on adhesions. The rats were sacrificed on day 10 and colon tissues and blood samples were collected for macroscopic, histopathological, and biochemical analysis.
Results: In our study, E-selectin, P-selectin, TNF-α and IL-1 levels were statistically significantly lower in the MSC group than the control group, while the sham group has the lowest levels. In both the macroscopic and histopathological analyses (Zühlke’s scale), the least amount of adhesion was observed in the Sham group. In addition, although there was less adhesion in the MSC group than the control group, the difference did not reach statistical significance.
Conclusion: Topical MSC application immediately after surgery suppresses the inflammatory process. However it was found to be ineffective in histopathological and macroscopic examinations performed on the 10th day.

#Mesenchymal stem cell #Adhesions #Morphological and microscopic findings #Selectins

참고문헌 (24)

참고문헌 신청

Drollette CM / 1992 / Pathophysiology of pelvic adhesions. Modern trends in pre venting infertility / J Reprod Med 37:107~121

diZerega GS / 1994 / Contemporary adhesion preven tion / Fertil Steril 61:219~235

diZerega GS / 2001 / Peritoneal repair and post-surgical adhesion formation / Hum Reprod Update 7:547~555

Brunschwig A / 1953 / Regeneration of peritoneum: experimental obser vations and clinical experience in radical re sections of intra-abdominal cancer / XVth Congress of the Society of Interna tional Chirurgie :756~765

Johnson F R / 1962 / R epair o f parietal peritoneum / Br J Surg 49:653~660

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