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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 49 - 57 (9page)
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초록· 키워드
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Background: Korean Red Ginseng (KRG; Panax ginseng Meyer) is a widely used medicinal herb known to exert various immune modulatory functions. KRG and one of its purified components, ginsenoside Rg3, are known to possess anti-inflammatory activities. How they impact helper T cell-mediated responses is not fully explored. In this study, we attempted to evaluate the effect of KRG extract (KRGE) and ginsenoside Rg3 on Th1 cell responses.
Methods: Using well-characterized T cell in vitro differentiation systems, we examined the effects of KRGE or enhanced Rg3 on the Th1-inducing cytokine production from dendritic cells (DC) and the naïve CD4þ T cells differentiation to Th1 cells. Furthermore, we examined the change of Th1 cell population in the intestine after treatment of enhanced Rg3. The influence of KRGE or enhanced Rg3 on Th1 cell differentiation was evaluated by fluorescence-activated cell sorting, enzyme-linked immunosorbent assay, and quantitative real-time polymerase chain reaction.
Results: KRGE significantly inhibited the production level of IL-12 from DCs and subsequent Th1 cell differentiation. Similarly, enhanced Rg3 significantly suppressed the expression of interferon gamma (IFNγ) and T-bet in T cells under Th1-skewing condition. Consistent with these effects in vitro, oral administration of enhanced Rg3 suppressed the frequency of Th1 cells in the Peyer’s patch and lamina propria cells in vivo.
Conclusion: Enhanced Rg3 negatively regulates the differentiation of Th1 cell in vitro and Th1 cell responses in the gut in vivo, providing fundamental basis for the use of this agent to treat Th1-related diseases.
Methods: Using well-characterized T cell in vitro differentiation systems, we examined the effects of KRGE or enhanced Rg3 on the Th1-inducing cytokine production from dendritic cells (DC) and the naïve CD4þ T cells differentiation to Th1 cells. Furthermore, we examined the change of Th1 cell population in the intestine after treatment of enhanced Rg3. The influence of KRGE or enhanced Rg3 on Th1 cell differentiation was evaluated by fluorescence-activated cell sorting, enzyme-linked immunosorbent assay, and quantitative real-time polymerase chain reaction.
Results: KRGE significantly inhibited the production level of IL-12 from DCs and subsequent Th1 cell differentiation. Similarly, enhanced Rg3 significantly suppressed the expression of interferon gamma (IFNγ) and T-bet in T cells under Th1-skewing condition. Consistent with these effects in vitro, oral administration of enhanced Rg3 suppressed the frequency of Th1 cells in the Peyer’s patch and lamina propria cells in vivo.
Conclusion: Enhanced Rg3 negatively regulates the differentiation of Th1 cell in vitro and Th1 cell responses in the gut in vivo, providing fundamental basis for the use of this agent to treat Th1-related diseases.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusions
References
참고문헌 (56)
참고문헌 신청
Yun TK / 2001 / Brief introduction of Panax ginseng C.A. Meyer / J Korean Med Sci 16(Suppl):S3~S5
Seung-Hoon Baek / 2012 / Recent Methodology in Ginseng Analysis / Journal of Ginseng Research 36(2):119~134
Park BG / 2013 / Potentiation of antioxidative and anti-inflammatory properties of cultured wild ginseng root extract through probiotic fermentation / J Pharm Pharmacol 65:457~464
Wang L / 2015 / Structural and antiinflammatory characterization of a novel neutral polysaccharide from North American ginseng(Panax quinquefolius) / Int J Biol Macromol :7412~7417
박은영 / 2012 / Increase in Insulin Secretion Induced by Panax ginseng Berry Extracts Contributes to the Amelioration of Hyperglycemia in Streptozotocininduced Diabetic Mice / Journal of Ginseng Research 36(2):153~160
Seung-Hoon Baek / 2012 / Recent Methodology in Ginseng Analysis / Journal of Ginseng Research 36(2):119~134
Park BG / 2013 / Potentiation of antioxidative and anti-inflammatory properties of cultured wild ginseng root extract through probiotic fermentation / J Pharm Pharmacol 65:457~464
Wang L / 2015 / Structural and antiinflammatory characterization of a novel neutral polysaccharide from North American ginseng(Panax quinquefolius) / Int J Biol Macromol :7412~7417
박은영 / 2012 / Increase in Insulin Secretion Induced by Panax ginseng Berry Extracts Contributes to the Amelioration of Hyperglycemia in Streptozotocininduced Diabetic Mice / Journal of Ginseng Research 36(2):153~160
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UCI(KEPA) : I410-ECN-0101-2019-524-000425287