지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.6
- 수록면
- 475 - 481 (7page)
이용수
초록· 키워드
오류제보하기
Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined.
Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis.
Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolismwith elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling.
Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.
Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis.
Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolismwith elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling.
Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (28)
참고문헌 신청
Rolfe DF / 1997 / Cellular energy utilization and molecular origin of standard metabolicrate in mammals / Physiol Rev 77:731~758
Holloszy JO / 1967 / Biochemical adaptations in muscle. Effects of exercise in mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle / J Biol Chem 242:2278~2282
Gundersen K / 1998 / Determination of muscle contractile properties: the importance of the nerve / Acta Physiol Scand 162:333~341
Willis LH / 2012 / Effects of aerobic and/or resistance training on body mass and fat mass in overweight or obese adults / J Appl Physiol 113:1831~1837
Wakahara T / 2013 / Nonuniform muscle hypertrophy : itsrelation to muscle activation in training session / Med Sci Sports Exerc 45:2158~2165
Holloszy JO / 1967 / Biochemical adaptations in muscle. Effects of exercise in mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle / J Biol Chem 242:2278~2282
Gundersen K / 1998 / Determination of muscle contractile properties: the importance of the nerve / Acta Physiol Scand 162:333~341
Willis LH / 2012 / Effects of aerobic and/or resistance training on body mass and fat mass in overweight or obese adults / J Appl Physiol 113:1831~1837
Wakahara T / 2013 / Nonuniform muscle hypertrophy : itsrelation to muscle activation in training session / Med Sci Sports Exerc 45:2158~2165
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