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논문 기본 정보

자료유형
학술저널
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대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제1권 제4호
발행연도
2008.1
수록면
189 - 195 (7page)

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Objectives. Toll-like receptors (TLRs) detect microbial infections and they can directly induce innate host defense responses. TLR 2 has been shown to be primarily involved in the recognition of peptidoglycans and lipoteichoic acid of gram positive bacteria. TLR 4 recognizes lipopolysaccharides and lipoteichoic acids from both gram-negative and grampositive bacteria. Both mutations lead a reduced capacity to elicit inflammation and they increase the risk for gram-positive and negative infections. This study was performed to investigate the expressions of TLR 2 and 4 and their mutations in patients suffering with otitis media and middle ear effusion. Methods. Middle ear fluid samples were collected from 40 otitis media effusion (OME) patients who had ventilating tubes inserted. Bacteria in the effusion fluid were detected by standard bacterial culture. The secreted IgG, IgA and IgM were measured by Enzyme-linked immunosorbent assay. TLR 2 and 4 were assessed by performing RT-PCR. The genomic DNA from each patient was isolated from the middle ear fluid samples that were collected from 60 OME patients, and the presence of mutations was determined by performing restriction digestion and DNA sequencing analysis. Results. Among the 40 middle ear fluid samples, bacteria were detected in 13 middle ear fluid samples. The amounts of IgM, IgA, and IgG were 151.20±60.94 ng/mL, 21.59±7.96 ng/mL and 11.55±16.98 ng/mL, respectively. TLR 2 and 4 were expressed in the middle ear fluid and the expression of TLR 2 was higher than that of TLR 4. However, there was no correlation between the expressions of TLR 2 and 4, and the concentration of immunoglobulin or the presence of bacteria (P>0.05). There ware no mutations of TLR 2 (Arg753Gln, Arg677Trp) and TLR 4 (Asp299Gly, Thr399Ile). Conclusion. TLR 2 and 4 were expressed in all the middle ear fluid samples of OME, but the mutations of TLR 2 and 4 were not detected. TLR 2 and 4 may play a vital role in the immunological responses of patients with OME.
#Otitis media #Toll-like receptor #Mutation

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참고문헌 (25)

참고문헌 신청
Agnese DM / 2002 / Human toll-like receptor 4 mutations but not CD14 polymorphisms are associated with an increased risk of gram-negative infections / J Infect Dis 186 (10) google schola Akira S / 2001 / Toll-like receptors: critical proteins linking innate and acquired immunity / Nat Immunol 2 (8) : 675 ~ 680 google schola Aliprantis AO / 1999 / Cell activation and apoptosis by bacterial lipoproteins through tolllike receptor-2 / Science (285) : 736 ~ 739 google schola Arbour NC / 2000 / mutations are associated with endotoxin hyporesponsiveness in humans / Nat Genet 25 (2) : 187 ~ 191 google schola Chaudhary PM / 1998 / Cloning and characterization of two Toll/Interleukin-1 receptorlike genes TIL3 and TIL4: evidence for a multi-gene receptor family in humans / Blood 91 google schola

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