지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 513 - 523 (11page)
이용수
초록· 키워드
오류제보하기
Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 µg/µL cDNA and 25 µL effectene and cell viabilities were also satisfactory under this condition, and the over-production of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced di-abetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non-diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable isles and increased VEGF-transfected grafts vascularization were observed in renal capsules. These studies support the usefulness of VEGF-transfected islet delivery using a cationic lipid reagent to achieve euglycemia using a minimal number of islets.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
/ / . Assessment of islet cell viability using fluorescentdyes. Diabetologia 1987 30 : 812 ~ 6
/ / Ahmed A. Activation of vascular endothelial growth factorreceptor-1 sustains angiogenesis and Bcl-2 expression viathe phosphatidylinositol 3-kinase pathway in endothelial
/ / Aiello LP. Vascular endothelial growthfactor in ocular neovascularization and proliferative diabeticretinopathy diabetes. Metab Rev 1997 : 37 ~ 50
/ / Benjamin LE. Placentalgrowth factor is a survival factor for tumor endothelial cellsand macrophages. Cancer Res 2002 : 2749 ~ 52
/ / Bromberg JS. Adenovirus transductioninduces expression of multiple chemokines andchemokine receptors in murine β-cells and pancreatic islets.Am J Transplant 2003 : 1230
/ / Ahmed A. Activation of vascular endothelial growth factorreceptor-1 sustains angiogenesis and Bcl-2 expression viathe phosphatidylinositol 3-kinase pathway in endothelial
/ / Aiello LP. Vascular endothelial growthfactor in ocular neovascularization and proliferative diabeticretinopathy diabetes. Metab Rev 1997 : 37 ~ 50
/ / Benjamin LE. Placentalgrowth factor is a survival factor for tumor endothelial cellsand macrophages. Cancer Res 2002 : 2749 ~ 52
/ / Bromberg JS. Adenovirus transductioninduces expression of multiple chemokines andchemokine receptors in murine β-cells and pancreatic islets.Am J Transplant 2003 : 1230
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Transplantation of betacellulin-transduced islets improves glucose intolerance in diabetic mice
Experimental and Molecular Medicine
2014 .01
-
Effect of nicotinamide on early graft failure following intraportal islet transplantation
Experimental and Molecular Medicine
2009 .01
-
Regulatory T Cells Promote Pancreatic Islet Function and Viability via TGF-1 in vitro and in vivo
대한임상검사과학회지
2018 .01
-
Bilirubin protects grafts against nonspecific inflammationinduced injury in syngeneic intraportal islet transplantation
Experimental and Molecular Medicine
2010 .01
-
Adenoviral mediated hepatocyte growth factor gene attenuates hyperglycemia and beta cell destruction in overt diabetic mice
Experimental and Molecular Medicine
2003 .01
-
TGF-β1 induces mouse dendritic cells to express VEGF and its receptor (Flt-1) under hypoxic conditions
Experimental and Molecular Medicine
2010 .01
-
Angiotensin II receptor blocker attenuates overexpression of vascular endothelial growth factor in diabetic podocytes
Experimental and Molecular Medicine
2004 .01
-
A serum-stable branched dimeric anti-VEGF peptide blocks tumor growth via anti-angiogenic activity
Experimental and Molecular Medicine
2010 .01
-
An antioxidant modulates expression of receptor activator of NF-κB in asthma
Experimental and Molecular Medicine
2006 .01
-
Combined blockade of HER2 and VEGF exerts greater growth inhibition of HER2-overexpressing gastric cancer xenografts than individual blockade
Experimental and Molecular Medicine
2013 .01
-
Prolonged survival of islet allografts in mice treated withrosmarinic acid and anti-CD154 antibody
Experimental and Molecular Medicine
2008 .01
-
An Ecological Study on the Vegetation in Saryang-Islet
한국동물학회 학술대회
1997 .01
-
High efficiency transduction of human VEGF165 into human skeletal myoblasts: in vitro studies
Experimental and Molecular Medicine
2003 .01
-
A novel cationic liposome reagent for gene transfer with low toxicity
한국생명과학회 심포지움
2006 .09
-
Synthesis of Novel Cholesterol Based Cationic Lipids, Transfection Biology of Human Cell line
한국생명과학회 심포지움
2008 .10
-
당뇨병성 망막증에 있어서 Captopril에 의한 VEGF 발현 저하 효과
한국현미경학회지
2009 .01
-
The discovery of placenta growth factor and its biological activity
Experimental and Molecular Medicine
2012 .01
-
Improved expression by cytomegalovirus promoter/enhancer and behavior of vascular endothelial growth factor gene after myocardial injection of naked DNA
Experimental and Molecular Medicine
2002 .01
-
Tissue microarray를 이용한 사이모신 베타4(Thymosin β4)와 vascular endothelial cell growth factor (VEGF)의 정상 인간 조직 발현 양상 연구
생명과학회지
2009 .12
-
해양심층수 섭취가 STZ로 유발된 제 1형 당뇨 생쥐의 혈당치 및 췌도에 미치는 영향
생명과학회지
2009 .07
이 논문의 저자 정보
최근 본 자료
댓글(0)
0