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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제2호
- 발행연도
- 2008.1
- 수록면
- 295 - 298 (4page)
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초록· 키워드
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Creatine kinase (CK; E.C. 2.7.3.2) is an important enzymethat catalyzes the reversible transfer of a phosphorylgroup from ATP to creatine in energy homeostasis. Thebrain-type cytosolic isoform of creatine kinase (BB-CK),which is found mainly in the brain and retina, is a keyenzyme in brain energy metabolism, because high-energyphosphates are transfered through the creatine kinase/phosphocreatine shuttle system. The recombinant humanBB-CK protein was overexpressed as a soluble form inEscherichia coli and crystallized at 22oC using PEG 4000as a precipitant. Native X-ray diffraction data were collectedto 2.2 Å resolution using synchrotron radiation. Thecrystals belonged to the tetragonal space group P43212,with cell parameters of a=b=97.963, c=164.312 Å, and α=β=γ=90o. The asymmetric unit contained two molecules ofCK, giving a crystal volume per protein mass (Vm) of1.80 Å3 Da-1 and a solvent content of 31.6%.
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참고문헌 (20)
참고문헌 신청
Abraham, M. R. / 2002 / Coupling of cell energetics with membrane metabolic sensing. Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knoc
Aksenov, M. Y. / 1997 / The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders: Alzheimer’s and Pick’s disease. Exp / Neurol
Crawford, R. M. / 2002 / Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo / FASEB 16 : 102 ~ 104
David, S. / 1998 / Abnormal properties of creatine kinase in Alzheimer’s disease brain: Correlation of reduced enzyme activity and active site photolabeling with aberrant cy
Eder, M. / 1999 / Crystal structure of brain-type creatine kinase at 1.41 A resolution / Protein Sci 8 : 2258 ~ 2269
Aksenov, M. Y. / 1997 / The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders: Alzheimer’s and Pick’s disease. Exp / Neurol
Crawford, R. M. / 2002 / Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo / FASEB 16 : 102 ~ 104
David, S. / 1998 / Abnormal properties of creatine kinase in Alzheimer’s disease brain: Correlation of reduced enzyme activity and active site photolabeling with aberrant cy
Eder, M. / 1999 / Crystal structure of brain-type creatine kinase at 1.41 A resolution / Protein Sci 8 : 2258 ~ 2269
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