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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제1호
- 발행연도
- 2015.1
- 수록면
- 81 - 88 (8page)
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초록· 키워드
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This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermentation phase, and a low oxygen supply level in the later phase was carried out. As a consequence, a maximum L-LDH activity of 95.6 U/ml was obtained in the recombinant strain, which was over 4-fold higher than that of the initial strain. Under the TOS for L. casei (pMG-ldhL), the maximum lactic acid concentration of 159.6 g/l was obtained in 36 h, corresponding to a 62.8% increase. The results presented here provide a novel way to regulate the metabolic flux of L. casei for lactic acid production in different fermentation stages, which is available to enhance organic acid production in other strains.
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참고문헌 (22)
참고문헌 신청
Abdullah AM / 2010 / Improvement of multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 under conditions of thermal stress by heterologous expre
Alcantara C / 2014 / Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance / Appl. Environ. Microbiol 80 : 1650 ~ 1659
Arai K / 2010 / Active and inactive state structures of unliganded Lactobacillus casei allosteric Llactate dehydrogenase / Proteins 78 : 681 ~ 694
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Chen J / 2013 / Oxidative stress at high temperatures in Lactococcus lactis due to an insufficient supply of riboflavin / Appl. Environ. Microbiol 79 : 6140 ~ 6147
Alcantara C / 2014 / Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance / Appl. Environ. Microbiol 80 : 1650 ~ 1659
Arai K / 2010 / Active and inactive state structures of unliganded Lactobacillus casei allosteric Llactate dehydrogenase / Proteins 78 : 681 ~ 694
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Chen J / 2013 / Oxidative stress at high temperatures in Lactococcus lactis due to an insufficient supply of riboflavin / Appl. Environ. Microbiol 79 : 6140 ~ 6147
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