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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Cardiovascular and cerebrovascular diseases seriously harm human health, and Bifidobacterium is the most beneficial probiotic in the gastrointestinal tract of humans. This work aimed to screen cholesterol-lowering Bifidobacterium from Guizhou Xiang Pig and evaluate its tolerance to oxygen, acid, and bile. Twenty-seven aerotolerant strains with similar colony to Bifidobacterium were isolated through incubation at 37℃ in 20% (v/v) CO<sub>2</sub>-80% (v/v) atmospheric air by using Mupirocin lithium modified MRS agar medium, modified PTYG with added CaCO<sub>3</sub>, and modified PTYG supplemented with X-gal. Ten strains with cholesterol-lowering rates above 20% (w/w) were used for further screening. The selected strains’ tolerance to acid and bile was then determined. A combination of colony and cell morphology, physiological, and biochemical experiments, as well as 16S rRNA gene-sequence analysis, was performed. Results suggested that BZ25 with excellent characteristics of high cholesterol-removal rate of 36.32% (w/w), as well as tolerance to acid and bile, was identified as Bifidobacterium animalis subsp. lactis. To further evaluate Bifidobacterium BZ25’s growth characteristic and tolerance to oxygen, culture experiments were performed in liquid medium and an agar plate. Findings suggested that BZ25 grew well both in environmental 20% (v/v) CO<sub>2</sub>-80% (v/v) atmospheric air and in 100% atmospheric air because BZ25 reached an absorbance of 1.185 at 600 nm in 100% atmospheric air. Moreover, BZ25 was aerotolerant and can grow in an agar medium under the environmental condition of 100% atmospheric air. This study can lay a preliminary foundation for the potential industrial applications of BZ25.
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참고문헌 (23)
참고문헌 신청
Ahn, J. / 2001 / Physiological responses of oxygen-tolerant anaerobic Bifidobacterium longum under oxygen / J. Microbiol. Biotechnol 11:443~451
Andriantsoanirina, V. / 2013 / Tolerance of Bifidobacterium human isolates to bile, acid and oxygen / Anaerobe 21:39~42
Cai, Y. / 1974 / Bifidobacterium lactis meile, et al. 1997 is a subjective synonym of Bifidobacterium animalis (mitsuoka 1969) scardovi and trovatelli 1974 / Microbiol. Immunol 4:815~820
Oh, C. Y. / 2000 / Cholesterol lowering effect of lactic acid bacteria isolated from the human intestine / Korean J. Vet. Public Health 24:181~188
Dunne, C. / 2001 / Adaptation of bacteria to the intestinal niche:probiotics and gut disorder / Inflamm. Bowel. Dis 7:136~145
Andriantsoanirina, V. / 2013 / Tolerance of Bifidobacterium human isolates to bile, acid and oxygen / Anaerobe 21:39~42
Cai, Y. / 1974 / Bifidobacterium lactis meile, et al. 1997 is a subjective synonym of Bifidobacterium animalis (mitsuoka 1969) scardovi and trovatelli 1974 / Microbiol. Immunol 4:815~820
Oh, C. Y. / 2000 / Cholesterol lowering effect of lactic acid bacteria isolated from the human intestine / Korean J. Vet. Public Health 24:181~188
Dunne, C. / 2001 / Adaptation of bacteria to the intestinal niche:probiotics and gut disorder / Inflamm. Bowel. Dis 7:136~145
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