Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis

Yue Wang; An Ma; Xiao-Long Liu; Praphathip Eamsobhana; Xiao-Xian Gan

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저자정보: Yue Wang (Hangzhou Medical College) An Ma (Hangzhou Medical College) Xiao-Long Liu (Hangzhou Medical College) Praphathip Eamsobhana (Mahidol University) Xiao-Xian Gan (Hangzhou Medical College)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.59 No.3

발행연도: 2021.6

수록면: 257 - 263 (7page)

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Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

#Gnathostomiasis #diagnosis #DIGFA #IgG4

참고문헌 (33)

참고문헌 신청

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