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논문 기본 정보

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학술저널
저자정보
Lee Jong-Uk (Division of Allergy and Respiratory Diseases Department of Internal Medicine Soonchunhyang Universi) 이현주 (순천향대학교) Kim Ji-Na (Division of Allergy and Respiratory Diseases Department of Internal Medicine Soonchunhyang Universi) 김민경 (순천향대학교부속부천병원) 김성렬 (순천향대학교) 장헌수 (순천향대학교) 박춘식 (순천향대학교) 박종숙 (순천향대학교)
저널정보
대한의학회 Journal of Korean Medical Science Journal of Korean Medical Science Vol.35 No.32
발행연도
2020.1
수록면
1 - 13 (13page)

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Background: Exposure to ozone (O3) induces neutrophilic inflammation and goblet cell hyperplasia in humans and experimental animals. Because the solute carrier family 26-member 4 (Slc26a4; pendrin) gene induces mucin production and intraluminal acidification in the airways, it was hypothesized to be a key molecule in O3-induced airway injury. Thus, we evaluated the role of Slc26a4 and the protective effects of ammonium chloride (NH4Cl) in O3-induced airway injury in mice. Methods: Six-week-old female BALB/c mice were exposed to filtered air or O3 for 21 days (2 ppm for 3 hr/day). NH4Cl (0, 0.1, 1, and 10 mM) was administered intratracheally into the airways. Airway resistance was measured using a flexiVent system, and bronchoalveolar lavage fluid (BALF) cells were differentially counted. Slc26a4 and Muc5ac proteins and mRNA were measured via western blotting, real-time polymerase chain reaction, and immunostaining. Tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-17, IL-1β, and caspase-1 were analyzed via western blotting. Results: The levels Slc26a4 protein and mRNA significantly increased in lung tissues from Day 7 to Day 21 of O3 exposure, with concomitant increases in lung resistance, numbers of goblet cells in lung tissues, and inflammatory cells and thiocyanate (SCN− ) levels in BALF in a time-dependent manner. Treatment with NH4Cl significantly reduced these changes to levels similar to those of sham-treated mice, with a concomitant reduction of Slc26a4 proteins in lung lysates and SCN− levels in BALF. Slc26a4 protein was co-expressed with muc5ac protein in the bronchial epithelium, as indicated by immunofluorescence staining. NH4Cl treatment also significantly attenuated the O3-induced increases in IFN-γ, TNF-α, IL-17, IL-1β, and p20- activated caspase-1. Conclusion: Slc26a4 may be involved in O3-induced inflammatory and epithelial changes in the airways via activation of the inflammasome and the induction of IL-17 and IFN-γ. NH4Cl shows a potential as a therapeutic agent for controlling O3-induced airway inflammation and epithelial damage by modulating Slc26a4 expression.
#Slc26a4 #AHR #Ozone #Neutrophilic Inflammation #Muc5ac #NH4Cl

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참고문헌 (41)

참고문헌 신청
Lin S / 2008 / Chronic exposure to ambient ozone and asthma hospital admissions among children / Environ Health Perspect 116(12):1725~1730 google schola Galán I / 2003 / Short-term effects of air pollution on daily asthma emergency room admissions / Eur Respir J 22(5):802~808 google schola Medina-Ramón M / 2006 / The effect of ozone and PM10 on hospital admissions for pneumonia and chronic obstructive pulmonary disease : a national multicity study / Am J Epidemiol 163(6):579~588 google schola Ramadour M / 2000 / Prevalence of asthma and rhinitis in relation to long-term exposure to gaseous air pollutants / Allergy 55(12):1163~1169 google schola Wang T / 2001 / Ozone and related gaseous pollutants in the boundary layer of eastern China : overview of the recent measurements at a rural site / Geophys Res Lett 28(12):2373~2376 google schola

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