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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 627 - 636 (10page)
- DOI
- 10.14348/molcells.2021.2254
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초록· 키워드
오류제보하기
The three-dimensional organization of chromatin and its time-dependent changes greatly affect virtually every cellular function, especially DNA replication, genome maintenance, transcription regulation, and cell differentiation. Sequencing-based techniques such as ChIP-seq, ATAC-seq, and Hi-C provide abundant information on how genomic elements are coupled with regulatory proteins and functionally organized into hierarchical domains through their interactions. However, visualizing the time-dependent changes of such organization in individual cells remains challenging. Recent developments of CRISPR systems for site-specific fluorescent labeling of genomic loci have provided promising strategies for visualizing chromatin dynamics in live cells. However, there are several limiting factors, including background signals, off-target binding of CRISPR, and rapid photobleaching of the fluorophores, requiring a large number of target-bound CRISPR complexes to reliably distinguish the target-specific foci from the background. Various modifications have been engineered into the CRISPR system to enhance the signal-to-background ratio and signal longevity to detect target foci more reliably and efficiently, and to reduce the required target size. In this review, we comprehensively compare the performances of recently developed CRISPR designs for improved visualization of genomic loci in terms of the reliability of target detection, the ability to detect small repeat loci, and the allowed time of live tracking. Longer observation of genomic loci allows the detailed identification of the dynamic characteristics of chromatin. The diffusion properties of chromatin found in recent studies are reviewed, which provide suggestions for the underlying biological processes.
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참고문헌 (87)
참고문헌 신청
Agarwal, P. / 2017 / Chromatin Regulation and Dynamics / Academic Press :275~302
Anton, T. / 2014 / Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR / Cas system / Nucleus 5:163~172
Barutcu, A. R. / 2015 / Chromatin interaction analysis reveals changes in small chromosome and telomere clustering between epithelial and breast cancer cells / Genome Biol 16:214
Beliveau, B. J. / 2012 / Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes / Proc. Natl. Acad. Sci. U. S. A 109:21301~21306
Bickmore, W. A / 2013 / The spatial organization of the human genome / Annu. Rev. Genomics Hum. Genet 14:67~84
Anton, T. / 2014 / Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR / Cas system / Nucleus 5:163~172
Barutcu, A. R. / 2015 / Chromatin interaction analysis reveals changes in small chromosome and telomere clustering between epithelial and breast cancer cells / Genome Biol 16:214
Beliveau, B. J. / 2012 / Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes / Proc. Natl. Acad. Sci. U. S. A 109:21301~21306
Bickmore, W. A / 2013 / The spatial organization of the human genome / Annu. Rev. Genomics Hum. Genet 14:67~84
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